Differential gene expression in Anopheles stephensi following infection with drug-resistant Plasmodium yoelii.
Differential gene expression in Anopheles stephensi following infection with drug-resistant Plasmodium yoelii.
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耐药约氏疟原虫感染史氏按蚊后差异基因表达
DOI：
10.1186/s13071-017-2326-y
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发表时间：
2017-08-29
影响因子：
3.2
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhang J;Huang J;Zhu F;Zhang J
背景：蚊虫传播抗药性寄生虫可能受到抗药性寄生虫生物适应性改变和蚊虫不同免疫反应或代谢变化的影响。在这一点上，人们对蚊子感染抗药性寄生虫后免疫力和代谢的变化知之甚少。为了解耐药疟原虫感染后斯氏按蚊基因表达的差异，我们对斯氏按蚊取食耐药或敏感的约氏疟原虫后的基因组转录谱进行了分析，观察了基因表达谱的变化，并鉴定了耐药疟原虫感染对斯氏按蚊基因表达的影响。通过stephensi基因转录，我们分析了疟原虫感染的三个主要过渡阶段：吸血后24小时、13天和19天。构建了6个cDNA文库（R-As 24 h、R-As 13 d、R-As 19 d、S-As 24 h、S-As 13 d和S-As 19 d），随后进行RNA测序。总共获得了大约5010万个原始读段、4790万个干净读段和7.18G干净碱基。通过差异表达基因（DEG）分析、GO富集DEG分析和KEGG功能分类，我们发现，An.斯氏疟原虫感染耐药约氏疟原虫NSM主要发生在血餐后13 d，此时子孢子通过血淋巴迁移。差异表达的基因包括一些重要的免疫反应和铁代谢途径的功能基因，如模式识别受体、JNK途径的调节因子、吞噬体途径的组分、黑化反应的调节因子、补体反应的激活因子、胰岛素信号级联成员、氧化应激和解毒蛋白。我们的研究表明，药物-耐药约氏疟原虫NSM对斯氏按蚊的转录本丰度水平有影响，主要是在子孢子通过血淋巴迁移期间血餐后13天，大多数差异表达的基因被下调。我们的研究结果强调，需要更好地了解选择压力，从这些差异表达的基因的抗药性疟原虫在蚊子和不同的传播模式的抗药性疟原虫按蚊。
Background:The transmission of drug-resistant parasites by the mosquito may be influenced by the altered biological fitness of drug-resistant parasites and different immune reactions or metabolic change in the mosquito. At this point, little is known about the variations in mosquito immunity and metabolism when mosquitoes are infected with drug-resistant parasites. To understand the differential gene expression in Anopheles following infection with drug-resistant Plasmodium, we conducted a genome-wide transcriptomic profiling analysis of Anopheles stephensi following feeding on mice with drug-resistant or drug-sensitive P. yoelii, observed changes in gene expression profiles and identified transcripts affected by the drug-resistant parasite.Results:To study the impact of drug-resistant Plasmodium infections on An. stephensi gene transcription, we analyzed the three major transition stages of Plasmodium infections: at 24 h and 13 and 19 days after blood-feeding. Six cDNA libraries (R-As24h, R-As13d, R-As19d,S-As24h, S-As13dand S-As19d) were constructed, and RNA sequencing was subsequently performed. In total, approximately 50.1 million raw reads, 47.9 million clean reads and 7.18G clean bases were obtained. Following differentially expressed gene (DEG) analysis, GO enrichment analysis of DEGs, and functional classification by KEGG, we showed that the variations in gene expression in An. stephensi infected by the drug-resistant P. yoelii NSM occurred mainly at 13 days after blood meal during sporozoite migration through the hemolymph. The differentially expressed genes included those functioning in some important immune reaction and iron metabolism pathways, such as pattern recognition receptors, regulators of the JNK pathway, components of the phagosome pathway, regulators of the melanization response, activators of complement reactions, insulin signaling cascade members, oxidative stress and detoxification proteins.Conclusions:Our study shows that drug-resistant P. yoelii NSM has an impact on the transcript abundance levels of An.stephensi mostly at 13 days after blood meal during sporozoite migration through the hemolymph and that most differentially expressed genes were downregulated. Our results highlight the need for a better understanding of selective pressures from these differentially expressed genes of the drug-resistant Plasmodium in the mosquito and the different transmission patterns of drug-resistant Plasmodium by Anopheles.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
3.8
作者：
Martinez-Barnetche, Jesus;Garcia Solache, Monica;Lanz-Mendoza, Humberto
通讯作者：
Lanz-Mendoza, Humberto
影响因子：
3.8
作者：
Paskewitz, Susan M.;Andreev, Olga;Shi, Lei
通讯作者：
Shi, Lei