Phospholipid composition and electric charge in healthy and cancerous parts of human kidneys.
Phospholipid composition and electric charge in healthy and cancerous parts of human kidneys.
复制标题
DOI：
10.1007/s00232-013-9554-7
复制
发表时间：
2013-05
影响因子：
2.4
通讯作者：
Kudelski, Jacek
中科院分区：
文献类型：
--
作者：
Szachowicz-Petelska, Barbara;Dobrzynska, Izabela;Skrodzka, Marta;Darewicz, Barbara;Figaszewski, Zbigniew A.;Kudelski, Jacek
关键词：
磷脂在自然界中普遍存在，并且是细胞膜的脂质双层所必需的。它们的结构和功能特性对于细胞的存活至关重要。在这项研究中，来自同一患者的健康人肾组织和癌性人肾组织的磷脂进行了比较，并特别参考了膜的电荷。本文介绍了一种简便、高效的磷脂含量分析正相法。本工作主要研究了无转移的pT 1期、G2级肾癌细胞膜中磷脂含量（PtdIns，磷脂酰肌醇; PtdSer，磷脂酰丝氨酸; PtdEtn，磷脂酰乙醇胺; PtdCho，磷脂酰胆碱）的变化。用电泳法测定了正常人和癌变人肾组织的表面电荷密度。在不同pH的溶液中进行测量。根据表面电荷密度作为pH值的函数，酸性（C TA）和碱性（C TB）官能团的浓度和它们与氢（K AH）或羟基（K BOH）离子的平均缔合常数进行了评估。癌细胞转化过程中磷脂总量增加，C TA和K BOH增加，而K AH和C TB较未转化的肿瘤细胞减少。
Phospholipids are ubiquitous in nature and are essential for the lipid bilayer of cell membranes. Their structural and functional properties are pivotal for the survival of the cell. In this study the phospholipids of healthy and cancerous human renal tissues from the same patients are compared with special reference to the electric charge of the membrane. A simple and highly effective normal-phase method is described for analyzing phospholipids content. This work is focused on changes of phospholipids content (PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; PtdEtn, phosphatidylethanoloamine; PtdCho, phosphatidylcholine) in cell membranes of renal cancer of pT1 stage, G2 grade, without metastasis. Surface charge density of healthy and cancerous human renal tissues was measured by electrophoresis. The measurements were carried out at various pH of solution. Depending on the surface charge density as a function of pH, acidic (C TA) and basic (C TB) functional group concentrations and their average association constants with hydrogen (K AH) or hydroxyl (K BOH) ions were evaluated. The process of cancer transformation was accompanied by an increase in total amount of phospholipids as well as an increase in C TA and K BOH, whereas K AH and C TB were decreased compared with unchanged tumor cells.
登录
查看更多内容
影响因子：
4.7
作者：
Hemminki, K;Jiang, YW;Lindblad, P
通讯作者：
Lindblad, P
影响因子：
6.6
作者：
Inagaki, T;Bhayani, SB;Kavoussi, LR
通讯作者：
Kavoussi, LR
DOI：
10.1016/s0360-3016(02)03928-7
发表时间：
2002-12-01
影响因子：
7
作者：
Ran, S;Thorpe, PE
通讯作者：
Thorpe, PE
影响因子：
1.9
作者：
Hoffmann, K;Blaudszun, J;Steinhart, H
通讯作者：
Steinhart, H
影响因子：
4.8
作者：
Zhao, J;Zhou, QS;Sims, PJ
通讯作者：
Sims, PJ