Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease.
Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease.
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DOI：
10.1002/eji.201243262
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发表时间：
2013-06
影响因子：
5.4
通讯作者：
Harari A
中科院分区：
文献类型：
--
作者：
Rozot V;Vigano S;Mazza-Stalder J;Idrizi E;Day CL;Perreau M;Lazor-Blanchet C;Petruccioli E;Hanekom W;Goletti D;Bart PA;Nicod L;Pantaleo G;Harari A
对结核分枝杆菌（Mtb）的保护性免疫仍知之甚少，Mtb特异性CD8 + T细胞的作用存在争议。在此，我们对326名潜伏性结核感染（LTBI）或活动性结核病（TB）患者的Mtb特异性CD8 + T细胞进行了广泛的表型和功能特征分析。在大多数（60%）结核病患者中检测到Mtb特异性CD8 + T细胞，而在少数（15%）LTBI受试者中也有检测到，但数量相似。LTBI受试者中的Mtb特异性CD8 + T细胞大多为TEMRA细胞（CD45RA + CCR7 - ），共表达2B4和CD160，而结核病患者中的Mtb特异性CD8 + T细胞大多为TEM细胞（CD45RA - CCR7 - ），表达2B4但缺乏PD - 1和CD160。两组的细胞因子谱没有显著差异。此外，Mtb特异性CD8 + T细胞表达低水平的穿孔素和颗粒溶素，但含有颗粒酶A和B。然而，体外扩增的Mtb特异性CD8 + T细胞表达穿孔素和颗粒溶素。最后，与肺结核患者相比，肺外结核患者中Mtb特异性CD8 + T细胞反应的检测频率较低。与肺结核患者相比，肺外结核患者中Mtb特异性CD8 + T细胞的增殖也更强。因此，Mtb感染的活动和临床表现与Mtb特异性CD8 + T细胞反应的不同特征相关。这些结果为Mtb与宿主免疫反应之间的相互作用提供了新的见解。
Protective immunity to Mycobacterium tuberculosis (Mtb) remains poorly understood and the role of Mtb-specific CD8+ T cells is controversial. Here we performed a broad phenotypic and functional characterization of Mtb-specific CD8+ T cells in 326 subjects with latent Mtb infection (LTBI) or active TB disease (TB). Mtb-specific CD8+ T cells were detected in most (60%) TB patients and few (15%) LTBI subjects but were of similar magnitude. Mtb-specific CD8+ T cells in LTBI subjects were mostly TEMRA cells (CD45RA+ CCR7− ), coexpressing 2B4 and CD160, and in TB patients were mostly TEM cells (CD45RA− CCR7− ), expressing 2B4 but lacking PD-1 and CD160. The cytokine profile was not significantly different in both groups. Furthermore, Mtb-specific CD8+ T cells expressed low levels of perforin and granulysin but contained granzymes A and B. However, in vitro-expanded Mtb-specific CD8+ T cells expressed perforin and granulysin. Finally, Mtb-specific CD8+ T-cell responses were less frequently detected in extrapulmonary TB compared with pulmonary TB patients. Mtb-specific CD8+ T-cell proliferation was also greater in patients with extrapulmonary compared with pulmonary TB. Thus, the activity of Mtb infection and clinical presentation are associated with distinct profiles of Mtb-specific CD8+ T-cell responses. These results provide new insights in the interaction between Mtb and the host immune response.
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影响因子：
82.9
作者：
Harari A;Rozot V;Bellutti Enders F;Perreau M;Stalder JM;Nicod LP;Cavassini M;Calandra T;Blanchet CL;Jaton K;Faouzi M;Day CL;Hanekom WA;Bart PA;Pantaleo G
通讯作者：
Pantaleo G
影响因子：
3.7
作者：
Caccamo N;Guggino G;Meraviglia S;Gelsomino G;Di Carlo P;Titone L;Bocchino M;Galati D;Matarese A;Nouta J;Klein MR;Salerno A;Sanduzzi A;Dieli F;Ottenhoff TH
通讯作者：
Ottenhoff TH
MTB衍生的抗原对人CD8+ T细胞的HLA-E依赖性表现。
影响因子：
15.3
作者：
Heinzel, Amy S;Grotzke, Jeff E;Lines, Rebecca A;Lewinsohn, Deborah A;McNabb, Andria L;Streblow, Daniel N;Braud, Veronique M;Grieser, Heather J;Belisle, John T;Lewinsohn, David M
通讯作者：
Lewinsohn, David M
DOI：
10.4049/jimmunol.1101122
发表时间：
2011-09-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Day CL;Abrahams DA;Lerumo L;Janse van Rensburg E;Stone L;O'rie T;Pienaar B;de Kock M;Kaplan G;Mahomed H;Dheda K;Hanekom WA
通讯作者：
Hanekom WA
影响因子：
15.9
作者：
Bruns, Heiko;Meinken, Christoph;Stenger, Steffen
通讯作者：
Stenger, Steffen