Mitochondrial genetic differentiation across populations of the malaria vector Anopheles lesteri from China (Diptera: Culicidae).
Mitochondrial genetic differentiation across populations of the malaria vector Anopheles lesteri from China (Diptera: Culicidae).
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DOI：
10.1186/1475-2875-10-216
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发表时间：
2011-08-03
期刊：
影响因子：
3
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Yang M;Ma Y;Wu J
雷氏按蚊是疟原虫的主要传播媒介。在中国中部。全面了解病媒种群结构和导致这种分化的过程，对于基于病媒的疟疾控制方案和查明病媒种群离散造成的疾病传播的异质性十分重要。没有足够的一个。有莱斯特里人的遗传数据。通过分析线粒体COII和Cytb基因序列的多态性，探讨了6个群体间的遗传变异和分化水平。来自中国的莱斯特里。mtDNA COII和Cytb基因分别有30个（4.37%）和21个（5.33%）多态位点。共获得31种COII单倍型和30种Cytb单倍型。mtDNA-COII的FST值范围为0.101 ~ 0.655，Cytb基因的FST值范围为0.029 ~ 0.231。AMOVA分析表明，两个基因的群体内变异率（65.83%，88.48%）大于群体间变异率（34.17%，11.52%）。除YN和HNB群体的Tajima's D值为负值外，其余群体的Tajima's D值和Fu's Fs值均为负值，表明群体中存在大量的低频突变，群体处于扩增过程中。遗传变异的水平在一个。lesteri种群高于其他种群。虽然这些结果可能表明相当程度的基因流动，但也可以假设其他解释，如历史人口扰动的影响。
Anopheles lesteri is a primary vector of Plasmodium spp. in central China. A complete understanding of vector population structure and the processes responsible for the differentiation is important to the vector-based malaria control programmes and for identifying heterogeneity in disease transmission as a result of discrete vector populations. There is no adequate An. lesteri population genetic data available. Polymorphism of sequence variations in mitochondrial COII and Cytb genes were assessed to explore the level of genetic variability and differentiation among six populations of An. lesteri from China. There were 30 (4.37%) and 21 (5.33%) polymorphic sites for mtDNA-COII and Cytb gene, respectively. Totally 31 COII and 30 Cytb haplotypes were obtained. The range of FST values was from 0.101 to 0.655 by mtDNA-COII, and 0.029 to 0.231 by Cytb gene. The analysis of molecular variance (AMOVA) showed that the percentage of variation within populations (65.83%, 88.48%) was greater than that among populations (34.17%, 11.52%) using both genes. The Tajima's D and Fu's Fs values were all negative, except Tajima's D values of YN and HNB populations, which suggest a large number of low-frequency mutations in populations and the populations were in expansion proceeding. Levels of genetic variation within An. lesteri populations were higher than among them. While these results may suggest considerable levels of gene flow, other explanations, such as the effect of historical population perturbations can also be hypothesized.
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影响因子：
4.9
作者：
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