Nonadditive and Asymmetric Allelic Expression of Growth Hormone in Hybrid Tilapia
Nonadditive and Asymmetric Allelic Expression of Growth Hormone in Hybrid Tilapia
复制标题
杂交罗非鱼生长激素的非加性和不对称等位基因表达
DOI：
10.3389/fgene.2019.00961
复制
发表时间：
2019-10
影响因子：
3.7
通讯作者：
Zhou Yi
中科院分区：
文献类型：
--
作者：
Zhong Huan;Zhang Xiaojin;Xu Qian;Yan Jinpeng;Han Zhuojun;Zheng Huifang;Xiao Jun;Tang Zhanyang;Wang Fenghua;Luo Yongju;Zhou Yi
杂交是水产养殖中常用的一种通过杂种优势改良种质的育种技术。然而，关键基因表达的调控，包括杂交事件开始时转录水平变化的细节，在很大程度上仍然不确定，特别是在硬骨鱼。本研究通过尼罗罗非鱼和蓝罗非鱼两个纯系的种间杂交，获得了一个杂交罗非鱼群体，并以此作为杂种优势的模型，对杂交罗非鱼群体中生长激素（GH）和等位基因特异性表达（ASE）的分子结果进行了追踪。在120天的生长试验中，杂交种与其亲本相比显示出生长活力。GH mRNA表达在垂体中唯一表达。高生长激素的表达被发现在混合比中亲值，在男性和女性，显示出非加性模式。我们确定了四个单核苷酸多态性位点之间的尼罗罗非鱼和蓝罗非鱼。随后，焦磷酸测序，我们发现不对称的等位基因表达在杂交后代中具有较高的母系等位基因转录比在男性和女性。禁食显着增加GH的表达在杂交，但不对称等位基因的表达不受喂养或禁食条件。最后，通过总表达量和ASE值分析了顺反效应，表明顺反效应促进了母本亚基因组在杂交罗非鱼中的表达，从而导致了GH在杂交罗非鱼中的优势表达。本研究结果首次阐明了生长势杂种鱼生长激素表达优势的概念及其形成机制。
Hybridization is a common breeding technique that can improve germplasm through heterosis in aquaculture. However, the regulation of key gene expression, including the details of transcriptional level changes at the beginning of hybridization events, remains largely undefined, especially in teleosts. In this study, by interspecies crossing between two pure lines of Nile tilapia and blue tilapia, we obtained a hybrid tilapia population as a model to elucidate heterosis, and we traced the molecular outcomes of growth hormone (GH) expression and allele-specific expression (ASE) in hybrids. The hybrids display growth vigor compared to their parents in the 120-day growth trial. GH mRNA expression was uniquely expressed in the pituitary. Higher GH expression was found in the hybrid than the midparent value, in both males and females, showing a nonadditive pattern. We identified four single-nucleotide polymorphism sites between Nile tilapia and blue tilapia. Subsequently, by pyrosequencing, we found asymmetric allelic expression in hybrids with higher maternal allelic transcript ratios in both males and females. Fasting significantly increased GH expression in hybrids, but asymmetric allelic expression was not affected by feeding or fasting conditions. Finally, we identified cis and trans effects via overall expression and ASE values in the hybrid, which showed that the cis and trans effects promoted the expression of maternal subgenome in the hybrid, contributing to the expression superiority of GH in hybrid tilapia. Taken together, the results of our study first illustrated the concept of GH expression superiority and its formation mechanism in hybrid fish with growth vigor.
登录
查看更多内容
染色质压实和组蛋白甲基化驱动在种间拟南芥杂交中的非腺基因表达。
影响因子：
12.3
作者：
Zhu W;Hu B;Becker C;Doğan ES;Berendzen KW;Weigel D;Liu C
通讯作者：
Liu C
影响因子：
3.7
作者：
Zhu F;Schlupp I;Tiedemann R
通讯作者：
Tiedemann R
DOI：
10.1073/pnas.1820513116
发表时间：
2019-03-19
影响因子：
11.1
作者：
Shao, Lin;Xing, Feng;Zhang, Qifa
通讯作者：
Zhang, Qifa
影响因子：
5.4
作者：
Guo, Mei;Rupe, Mary A.;Bowen, Ben
通讯作者：
Bowen, Ben
影响因子：
9.4
作者：
Cheng, Feng;Sun, Chao;Wang, Xiaowu
通讯作者：
Wang, Xiaowu