Altered chromatin compaction and histone methylation drive non-additive gene expression in an interspecific Arabidopsis hybrid.
Altered chromatin compaction and histone methylation drive non-additive gene expression in an interspecific Arabidopsis hybrid.
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染色质压实和组蛋白甲基化驱动在种间拟南芥杂交中的非腺基因表达。
DOI：
10.1186/s13059-017-1281-4
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发表时间：
2017-08-22
期刊：
影响因子：
12.3
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Zhu W;Hu B;Becker C;Doğan ES;Berendzen KW;Weigel D;Liu C
两个不同基因组的合并会导致杂种后代在表型上与父母双方都有很大的不同。在植物中，种间杂交在进化和物种形成中起着重要作用。此外，许多农业和园艺物种是由种间杂交而来的。然而，导致杂交种非加性表型新颖性的详细机制仍然难以捉摸。在拟南芥与拟南芥的种间杂交中，相对于亲本上调或下调的基因绝大多数来自拟南芥。在所有差异表达的拟南芥基因中，大多数在杂交中被下调。为了理解为什么亲本起源会影响这个系统中的基因表达，我们比较了杂种和亲本的染色质包装模式和表观基因组景观。我们发现，在杂交中，拟南芥的染色质变得更加致密，而拟南芥的染色质则没有。除了富转座子区域较高的CHH DNA甲基化外，亲本DNA甲基化模式和H3K27me3沉积在杂交种中基本不变。然而，富含H3K27me3标记的拟南芥基因在杂交后代和亲本之间的表达差异尤其明显。长期以来，人们一直怀疑基因组尺度的特性导致了亲本中一方或另一方基因对杂交的不同反应。我们的工作将拟南芥种间杂交基因表达的整体差异与整体染色质紧密性和H3K27me3组蛋白修饰联系起来。本文的在线版本（doi:10.1186/s13059-017- 1284 -4）包含补充材料，可供授权用户使用。
The merging of two diverged genomes can result in hybrid offspring that phenotypically differ greatly from both parents. In plants, interspecific hybridization plays important roles in evolution and speciation. In addition, many agricultural and horticultural species are derived from interspecific hybridization. However, the detailed mechanisms responsible for non-additive phenotypic novelty in hybrids remain elusive. In an interspecific hybrid between Arabidopsis thaliana and A. lyrata, the vast majority of genes that become upregulated or downregulated relative to the parents originate from A. thaliana. Among all differentially expressed A. thaliana genes, the majority is downregulated in the hybrid. To understand why parental origin affects gene expression in this system, we compare chromatin packing patterns and epigenomic landscapes in the hybrid and parents. We find that the chromatin of A. thaliana, but not that of A. lyrata, becomes more compact in the hybrid. Parental patterns of DNA methylation and H3K27me3 deposition are mostly unaltered in the hybrid, with the exception of higher CHH DNA methylation in transposon-rich regions. However, A. thaliana genes enriched for the H3K27me3 mark are particularly likely to differ in expression between the hybrid and parent. It has long been suspected that genome-scale properties cause the differential responses of genes from one or the other parent to hybridization. Our work links global chromatin compactness and H3K27me3 histone modification to global differences in gene expression in an interspecific Arabidopsis hybrid. The online version of this article (doi:10.1186/s13059-017-1281-4) contains supplementary material, which is available to authorized users.
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作者：
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发表时间：
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