Interspecies nuclear transfer embryos reconstructed from cat somatic cells and bovine ooplasm.
Interspecies nuclear transfer embryos reconstructed from cat somatic cells and bovine ooplasm.
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由猫体细胞和牛卵质重建的种间核移植胚胎。
DOI：
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发表时间：
2008
影响因子：
1.8
通讯作者：
K. Takeda
中科院分区：
文献类型：
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作者：
A. Thongphakdee;Shu‐Ichi Kobayashi;K. Imai;Y. Inaba;Mariko Tasai;T. Tagami;K. Nirasawa;T. Nagai;N. Saito;M. Techakumphu;K. Takeda
本研究旨在探讨家猫-牛异种体细胞核移植（iSCNT）重构胚的发育能力，并观察iSCNT重构胚的线粒体DNA（mtDNA）含量。使用混合品种家猫成纤维细胞作为供体细胞和去核牛卵母细胞作为受体细胞质产生iSCNT胚胎。当比较iSCNT胚胎和孤雌生殖牛胚胎的发育能力时，卵裂率和发育至8-细胞期的比率（分别为86.6vs.84.0%和32.2vs.36.2%）没有差异（P>0.05）。然而，与孤雌生殖胚胎发育到桑椹胚和囊胚阶段相反，没有iSCNT胚胎（0/202）发育超过8细胞阶段。对于mtDNA分析，随机选择1-细胞、2-细胞、4-细胞和8-细胞期的iSCNT胚胎。采用定量PCR对猫和牛mtDNA进行定量分析。在猫-牛iSCNT胚胎中，猫和牛mtDNA的水平在发育的每个阶段都不同。从1-细胞到8-细胞阶段，iSCNT胚胎中的猫mtDNA浓度是稳定的。8-细胞期iSCNT胚胎mtDNA含量显著低于4-细胞期（P<0.05）。在任何观察到的发育阶段（1至8细胞期），iSCNT胚胎中猫mtDNA的比例均无差异。总之，牛细胞质支持家猫细胞核发育通过8细胞阶段。家猫牛iSCNT胚胎的mtDNA基因型说明异质性的持久性，mtDNA含量的减少可能反映了在8细胞阶段的发育阻滞。
This study was conducted to investigate the developmental capacity of domestic cat-bovine reconstructed embryos via interspecies somatic cell nuclear transfer (iSCNT) and to observe the mitochondrial DNA (mtDNA) content of the iSCNT embryos. The iSCNT embryos were generated using mixed-breed domestic cat fibroblasts as donor cells and enucleated bovine oocytes as the recipient cytoplasm. When the developmental capacities of iSCNT embryos and parthenogenic bovine embryos were compared, there was no difference (P>0.05) in the rates of cleavage and development to the 8-cell stage (86.6 vs. 84.0% and 32.2 vs. 36.2%, respectively). However, in contrast to development of parthenogenic embryos to the morula and blastocyst stages, no iSCNT embryos (0/202) developed beyond the 8-cell stage. For mtDNA analysis, iSCNT embryos at the 1-cell, 2-cell, 4-cell and 8-cell stages were randomly selected. Both cat and bovine mtDNA quantification analysis were performed using quantitative PCR. The levels of both cat and bovine mtDNA in cat-bovine iSCNT embryos varied at each stage of development. The cat mtDNA concentration in the iSCNT embryos was stable from the 1-cell to 8-cell stages. The bovine mtDNA in the iSCNT embryos at the 8-cell stage was significantly lower than that at the 4-cell stage (P<0.05). No difference in the proportions of cat mtDNA in the iSCNT embryos was found in any of the observed developmental stages (1- through 8-cell stages). In conclusion, bovine cytoplasm supports domestic cat nucleus development through the 8-cell stage. The mtDNA genotype of domestic cat-bovine iSCNT embryos illustrates persistence of heteroplasmy, and the reduction in mtDNA content might reflect a developmental block at the 8-cell stage.
影响因子：
2.7
作者：
Katayama, Mika;Zhong, Zhisheng;Schatten, Heide
通讯作者：
Schatten, Heide
影响因子：
3.6
作者：
Dominko, T;Mitalipova, M;First, NL
通讯作者：
First, NL