Dataset of asymmetric giant unilamellar vesicles prepared via hemifusion: Observation of anti-alignment of domains and modulated phases in asymmetric bilayers.
Dataset of asymmetric giant unilamellar vesicles prepared via hemifusion: Observation of anti-alignment of domains and modulated phases in asymmetric bilayers.
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DOI：
10.1016/j.dib.2021.106927
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发表时间：
2021-04
期刊：
影响因子：
1.2
通讯作者：
Feigenson GW
中科院分区：
文献类型：
--
作者：
Enoki TA;Wu J;Heberle FA;Feigenson GW
本文提供的数据是对称巨单层囊泡（GUV）和非对称巨单层囊泡（aGUV）的共聚焦荧光图像。在这项工作中，使用半融合方法制备aGUV，并用两种不同的荧光染料标记，命名为TFPC和DiD。这两种染料显示出强烈的偏好的液体无序（Ld）相，而不是液体有序（Lo）相。有利于Ld相的这些染料的分区导致明亮的Ld相和黑暗的LO相域在对称的GUV中观察到的荧光显微镜。在对称囊泡中，内外小叶的亮区和暗区是对齐的。在aGUV中，荧光探针TFPC仅标记aGUV外小叶。在这里，我们展示了使用扫描荧光共聚焦显微镜获得的荧光显微照片的数据集。对于所选择的系统，TFPC和DiD的荧光信号显示aGUV上较亮结构域的反对齐。对于该数据集重要的是，TFPC和DiD分别具有以可见光谱的绿色和远红色区域为中心的荧光发射，并且染料的荧光发射带不重叠。该数据集是在与共同提交的工作（Enoki等人，2021）中报告的数据集相同的条件下收集的，其中大多数aGUV显示结构域对齐。此外，我们还展示了显示调制相位和宏域的GUV的显微照片。我们还比较了在GUV和aGUV中观察到的调制相位。对于这些数据集，我们使用共焦显微镜收集了一系列改变z位置的显微照片，称为z堆栈。在扫描显微镜Nikon Eclipse C2+（Nikon Instruments，梅尔维尔，NY）中收集图像。本文还提供了用于测量脂质浓度和制备具有准确脂质组分的GUV的其他样品。
The data provided with this paper are confocal fluorescence images of symmetric giant unilamellar vesicles (GUVs) and asymmetric giant unilamellar vesicles (aGUVs). In this work, aGUVs were prepared using the hemifusion method and are labelled with two different fluorescent dyes, named TFPC and DiD. Both dyes show strong preference for the liquid-disordered (Ld) phase instead of the liquid-ordered (Lo) phase. The partition of these dyes favoring the Ld phase leads to bright Ld phase and dark Lo phase domains in symmetric GUVs observed by fluorescence microscopy. In symmetric vesicles, the bright and the dark domains of the inner and the outer leaflets are aligned. In aGUVs, the fluorescent probe TFPC exclusively labels the aGUV outer leaflet. Here, we show a dataset of fluorescence micrographs obtained using scanning fluorescence confocal microscopy. For the system chosen, the fluorescence signal of TFPC and DiD show anti-alignment of the brighter domains on aGUVs. Important for this dataset, TFPC and DiD have fluorescence emission centered in the green and far-red region of the visible spectra, respectively, and the dyes’ fluorescence emission bands do not overlap. This dataset were collected in the same conditions of the dataset reported in the co-submitted work (Enoki, et al. 2021) where most of aGUVs show domains alignment. In addition, we show micrographs of GUVs displaying modulated phases and macrodomains. We also compare the modulated phases observed in GUVs and aGUVs. For these datasets, we collected a sequence of micrographs using confocal microscopy varying the z-position, termed a z-stack. Images were collected in a scanning microscope Nikon Eclipse C2+ (Nikon Instruments, Melville, NY). Additional samples used to measure the lipid concentrations and to prepare GUVs with accurate lipid fractions are also provided with this paper.
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影响因子：
3.4
作者：
Enokit, Thais A.;Feigenson, Gerald W.
通讯作者：
Feigenson, Gerald W.
DOI：
10.1016/j.bbamem.2021.183586
发表时间：
2021-06-01
期刊：
Biochimica et biophysica acta. Biomembranes
影响因子：
--
作者：
Enoki TA;Wu J;Heberle FA;Feigenson GW
通讯作者：
Feigenson GW
影响因子：
3.4
作者：
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通讯作者：
Feigenson, GW
影响因子：
3.4
作者：
KINGSLEY, PB;FEIGENSON, GW
通讯作者：
FEIGENSON, GW
影响因子：
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作者：
Konyakhina, Tatyana M.;Goh, Shih Lin;Feigenson, Gerald W.
通讯作者：
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