Flaxseed enriched diet-mediated reduction in ovarian cancer severity is correlated to the reduction of prostaglandin E(2) in laying hen ovaries.
Flaxseed enriched diet-mediated reduction in ovarian cancer severity is correlated to the reduction of prostaglandin E(2) in laying hen ovaries.
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DOI：
10.1016/j.plefa.2013.08.001
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发表时间：
2013-09
影响因子：
3
通讯作者：
Hales, Dale Buchanan
中科院分区：
文献类型：
--
作者：
Eilati, Erfan;Hales, Karen;Zhuge, Yan;Fricano, Kristine Ansenberger;Yu, Rui;van Breemen, Richard B.;Hales, Dale Buchanan
预防卵巢癌是减少这种致命疾病影响的最佳方法。产蛋母鸡是一个强大的自发性卵巢癌模型，重现了人类疾病。亚麻籽是欧米茄-3脂肪酸（OM-3FAs）和植物雌激素木脂素最丰富的植物来源，其饮食干预证明了通过靶向炎症前列腺素途径有效预防和改善卵巢癌的潜力。前列腺素E2（PGE 2）是最具促炎性的类花生酸，并且是环加氧酶（考克斯）的两种同工型考克斯-1和考克斯-2的下游产物之一。我们的目的是研究一年的亚麻籽补充对卵巢癌的影响，并将其影响与考克斯酶的表达和亚麻素的浓度相关联。白色来航母鸡饲喂10%亚麻籽强化日粮或标准日粮一年。卵巢癌的严重程度由大体病理学和组织学确定。分别采用免疫组化、Western blot、实时荧光定量PCR和LC-MS-MS检测卵巢中考克斯-1和考克斯-2的定位、蛋白和mRNA表达以及PGE 2和PGE 3的浓度。结果表明，与对照饮食喂养的母鸡相比，亚麻籽喂养的母鸡中晚期卵巢肿瘤显著减少。与卵巢癌严重程度降低相关的是，亚麻籽饲养母鸡卵巢中PGE 2浓度和考克斯-2表达减少。PGE 3浓度低于检测水平。结果表明，在正常卵巢中，考克斯-1定位于卵泡周围的颗粒细胞层和卵巢表面上皮（OSE），而考克斯-2蛋白定位于卵泡内的颗粒细胞层。考克斯-1和考克斯-2蛋白在卵巢癌组织中广泛表达。我们的研究结果表明，亚麻籽介导的降低母鸡卵巢癌的严重程度与亚麻籽喂养的母鸡卵巢中PGE 2的减少有关。这些发现可能为针对前列腺素生物合成的饮食干预预防和治疗卵巢癌的临床试验提供基础。
Prevention of ovarian cancer is the best approach for reducing the impact of this deadly disease. The laying hen is a robust model of spontaneous ovarian cancer that recapitulates the human disease. Dietary intervention with flaxseed, the richest vegetable source of omega-3 fatty acids (OM-3FAs) and phytoestrogen lignans, demonstrate the potential for effective prevention and amelioration of ovarian cancer by targeting inflammatory prostaglandin pathways. Prostaglandin E2 (PGE2) is the most pro-inflammatory ecoisanoid and one of the downstream products of two isoforms of cyclooxygenase (COX) enzymes: COX-1 and COX-2. Our objective was to investigate the effect of flaxseed supplementation for one year on ovarian cancer and correlate its effects to expression of COX enzymes and concentrations of prostaglandins. White Leghorn hens were fed 10% flaxseed-enriched or standard diet for one year. The severity of ovarian cancer was determined by gross pathology and histology. COX-1 and COX-2 localization and protein and mRNA expression and PGE2 and PGE3 concentrations in ovaries were measured by IHC, western blot, quantitative real-time PCR and LC-MS-MS, respectively. The results demonstrated a significant reduction in late stage ovarian tumors in the flaxseed-fed hens compared with the control diet-fed hens. In correlation with decreased ovarian cancer severity, concentrations of PGE2 and expression of COX-2 were diminished in ovaries of flaxseed-fed hens. PGE3 concentrations were below the level of detection. The results demonstrated that in normal ovaries, COX-1 was localized to the granulosa cell layer surrounding the follicles and ovarian surface epithelium (OSE) whereas COX-2 protein was localized to the granulosa cell layer in the follicle. Extensive COX-1 and COX-2 protein expression was found throughout the ovarian carcinoma. Our findings suggest that the flaxseed-mediated reduction in the severity of ovarian cancer in hens is correlated to the reduction in PGE2 in the ovaries of flaxseed-fed hens. These findings may provide the basis for clinical trials of dietary intervention targeting prostaglandin biosynthesis for the prevention and treatment of ovarian cancer.
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影响因子：
4.2
作者：
Hardman, WE
通讯作者：
Hardman, WE
影响因子：
3.7
作者：
Hales, Dale Buchanan;Zhuge, Yan;Bahr, Janice M.
通讯作者：
Bahr, Janice M.
影响因子：
4.8
作者：
Brecht, Kerstin;Weigert, Andreas;Bruene, Bernhard
通讯作者：
Bruene, Bernhard
影响因子：
11.5
作者：
Kobayashi, Naoko;Barnard, R. James;Aronson, William J.
通讯作者：
Aronson, William J.
影响因子：
2.9
作者：
BAUKNECHT, T;SIEGEL, A;ZAHRADNIK, HP
通讯作者：
ZAHRADNIK, HP