cDNA cloning of heat shock protein 90 gene and protein expression pattern in response to heavy metal exposure and thermal stress in planarian Dugesia japonica
cDNA cloning of heat shock protein 90 gene and protein expression pattern in response to heavy metal exposure and thermal stress in planarian Dugesia japonica
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涡虫热休克蛋白 90 基因的 cDNA 克隆及重金属暴露和热应激响应的蛋白表达模式
DOI：
10.1007/s11033-012-1552-9
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
De-Zeng Liu
中科院分区：
文献类型：
--
作者：
Guang-Wen Chen;Ke-Xue Ma;De-Zeng Liu
热休克蛋白90(HSP90)是一种含量丰富、高度保守的分子伴侣，在多种细胞应激反应中发挥重要作用。利用RACE技术首次克隆了日本涡虫DjHsp90(DjHsp90)的全长基因。该基因全长2,354个碱基，包括一个2,148个碱基的开放阅读框(ORF)，编码715个氨基酸的多肽，具有5个HSP90家族特征。我们对基因组DNA中的ORF序列进行了测序，发现Djhsp90基因结构中只有一个内含子(48bp)。我们用免疫印迹和免疫组织化学方法从蛋白水平分析了DjHsp90在重金属暴露和热应激条件下的表达模式。我们的结果表明，低剂量的重金属和较高的培养温度诱导了DjHsp90的表达，而高剂量的重金属和严重的热休克抑制了DjHsp90的表达。在重金属和热应激条件下，DjHsp90阳性细胞仅出现在从头部到尾部两侧表皮下的实质组织中。这些阳性细胞可能是传感器细胞，可以检测到外部环境的变化。我们的工作为研究行星动物的应激反应提供了基础数据。
Heat shock protein 90 (HSP90) is an abundant and highly conserved molecular chaperone, playing important roles in multiple cellular stress responses. The full-length cDNA of planarian Dugesia japonica Hsp90 (designated DjHsp90) was firstly cloned using rapid amplification of cDNA ends (RACE) techniques. It is 2,354 bp, including an open reading frame (ORF) of 2,148 bp encoding a polypeptide of 715 amino acids with all five HSP90 family signatures. We sequenced the ORF sequences from genomic DNA, and found only one intron (48 bp) existed in Djhsp90 gene structure. We used western blot and immunohistochemistry to analyze the expression pattern of DjHsp90 in response to heavy metal exposure and thermal stress at the protein level. Our results show that low doses of heavy metals and elevated culture temperature induced, but high doses of heavy metals and severe heat shock inhibited DjHsp90 expression. In response to heavy metals and thermal stress, DjHsp90-positive cells only appeared in the parenchymal tissue under epidermis cells along the bilateral from head to tail. These positive cells are presumably sensor cells that can detect external environment changes. Our work provides basic data for the study of stress responses in planarians.
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影响因子：
4
作者：
P. Csermely;T. Schnaider;Z. Prohászka;G. Nardai
通讯作者：
P. Csermely;T. Schnaider;Z. Prohászka;G. Nardai
DOI：
10.1002/9781119432401.ch9
发表时间：
2020-02
期刊：
Heat Stress Tolerance in Plants
影响因子：
--
作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
通讯作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
影响因子：
4.7
作者：
Wenbing Zhang;Chenglong Wu;K. Mai;Qiyong Chen;Wei Xu
通讯作者：
Wenbing Zhang;Chenglong Wu;K. Mai;Qiyong Chen;Wei Xu
DOI：
10.1016/j.cbpb.2005.09.005
发表时间：
2005-12-01
影响因子：
2.2
作者：
Cara, JB;Aluru, N;Vijayan, MM
通讯作者：
Vijayan, MM
影响因子：
4.8
作者：
Ahmed, R;Duncan, RF
通讯作者：
Duncan, RF