Extensive structural variations between mitochondrial genomes of CMS and normal peppers (Capsicum annuum L.) revealed by complete nucleotide sequencing.
Extensive structural variations between mitochondrial genomes of CMS and normal peppers (Capsicum annuum L.) revealed by complete nucleotide sequencing.
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DOI：
10.1186/1471-2164-15-561
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发表时间：
2014-07-04
期刊：
影响因子：
4.4
通讯作者：
Kang BC
中科院分区：
文献类型：
--
作者：
Jo YD;Choi Y;Kim DH;Kim BD;Kang BC
细胞质雄性不育（Cytoplasmic male不育，CMS）是由于线粒体基因组突变导致的不能产生功能性花粉的现象。通过对几种具有和不具有CMS的物种的线粒体基因组进行比较分析，揭示了基因组之间的结构差异，包括重组引起的广泛重排。研究人员获得了辣椒CMS系FS4401 （507,452 bp）和可育系济州（511,530 bp）的线粒体基因组全序列。对茄科辣椒和烟草的线粒体基因组进行比较分析，发现DNA重排广泛，非编码DNA保守性较差。在辣椒品系中，FS4401和济州的线粒体dna除了在FS4401中多了一个atp6基因拷贝（ψatp6-2）外，具有相同的蛋白质编码基因补体。在基因组结构方面，我们在两个线粒体基因组中发现了18个syntenic block，它们在每个基因组中都被重新排列。相比之下，FS4401和济州岛的同源区序列分别为30,380和17,847 bp。先前报道的CMS候选基因orf507和ψatp6-2位于FS4401特异性的最大序列片段的边缘。在这一区域，济州线粒体基因组中大量缺失或在不同位置发现的小序列片段被组合在一起。重复序列的结合和部分核苷酸的重叠表明，同源重组的广泛重排可能参与了该区域的进化。利用来自其他植物物种的mtDNA对进一步分析，揭示了cms相关基因周围DNA区域的共同特征。虽然CMS和雄性可育辣椒系线粒体基因组的大部分序列背景是共享的，但发现了大量的基因组重排。CMS候选基因位于高度重排的CMS特异性DNA区域边缘，靠近重复序列。这些特征在其他物种的cms相关基因中也被发现，这意味着cms相关基因的进化可能涉及一个共同的机制。本文的在线版本（doi:10.1186/1471-2164-15-561）包含补充材料，可供授权用户使用。
Cytoplasmic male sterility (CMS) is an inability to produce functional pollen that is caused by mutation of the mitochondrial genome. Comparative analyses of mitochondrial genomes of lines with and without CMS in several species have revealed structural differences between genomes, including extensive rearrangements caused by recombination. However, the mitochondrial genome structure and the DNA rearrangements that may be related to CMS have not been characterized in Capsicum spp. We obtained the complete mitochondrial genome sequences of the pepper CMS line FS4401 (507,452 bp) and the fertile line Jeju (511,530 bp). Comparative analysis between mitochondrial genomes of peppers and tobacco that are included in Solanaceae revealed extensive DNA rearrangements and poor conservation in non-coding DNA. In comparison between pepper lines, FS4401 and Jeju mitochondrial DNAs contained the same complement of protein coding genes except for one additional copy of an atp6 gene (ψatp6-2) in FS4401. In terms of genome structure, we found eighteen syntenic blocks in the two mitochondrial genomes, which have been rearranged in each genome. By contrast, sequences between syntenic blocks, which were specific to each line, accounted for 30,380 and 17,847 bp in FS4401 and Jeju, respectively. The previously-reported CMS candidate genes, orf507 and ψatp6-2, were located on the edges of the largest sequence segments that were specific to FS4401. In this region, large number of small sequence segments which were absent or found on different locations in Jeju mitochondrial genome were combined together. The incorporation of repeats and overlapping of connected sequence segments by a few nucleotides implied that extensive rearrangements by homologous recombination might be involved in evolution of this region. Further analysis using mtDNA pairs from other plant species revealed common features of DNA regions around CMS-associated genes. Although large portion of sequence context was shared by mitochondrial genomes of CMS and male-fertile pepper lines, extensive genome rearrangements were detected. CMS candidate genes located on the edges of highly-rearranged CMS-specific DNA regions and near to repeat sequences. These characteristics were detected among CMS-associated genes in other species, implying a common mechanism might be involved in the evolution of CMS-associated genes. The online version of this article (doi:10.1186/1471-2164-15-561) contains supplementary material, which is available to authorized users.
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