Differentiation of adipose-derived stem cells for tendon repair.
Differentiation of adipose-derived stem cells for tendon repair.
复制标题
脂肪干细胞的分化用于肌腱修复。
DOI：
10.1007/978-1-61737-960-4_32
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发表时间：
2011
影响因子：
--
通讯作者：
H. Mizuno
中科院分区：
文献类型：
--
作者：
A. Uysal;H. Mizuno
初次肌腱修复的目标是在活动时增加抗张强度。在几项研究中已经描述了使用间充质干细胞的肌腱修复和再生;然而，最近才考虑使用脂肪来源的干细胞（ASC）进行肌腱修复。为了建立一个合适的实验模型，用于使用ASC进行肌腱一期修复，本章描述了以下详细方法：（1）从脂肪组织中分离干细胞，（2）产生原发性肌腱损伤和修复模型，（3）通过测量拉伸强度评估功能恢复，（4）通过组织学和免疫组化分析探讨ASC介导的肌腱愈合机制。局部施用ASC至损伤部位加速肌腱修复，如拉伸强度的显著增加、ASC向腱细胞和内皮细胞的直接分化以及血管生成生长因子的增加所示。这些发现表明，ASCs可能对原发性肌腱修复有积极作用，并可能对未来的细胞治疗有用。
The goal of primary tendon repair is to increase tensile strength at the time of mobilization. Tendon repair and regeneration using mesenchymal stem cells have been described in several studies; however, the use of adipose derived stem cells (ASCs) for tendon repair has only recently been considered. In order to establish a suitable experimental model for the primary tendon repair using ASCs, this chapter describes the detailed methods for: (1) isolating stem cells from adipose tissue, (2) generation of a primary tendon injury and repair model, (3) evaluating functional restoration by measuring tensile strength, and (4) investigating the mechanisms involved in ASC-mediated tendon healing by histological and immunohistochemical analyses. Topical administration of ASCs to the site of injury accelerates tendon repair, as exhibited by a significant increase in tensile strength, direct differentiation of ASCs toward tenocytes and endothelial cells, and increases in angiogenic growth factors. These findings suggest that ASCs may have a positive effect on primary tendon repair and may be useful for future cell-based therapy.
影响因子：
--
作者：
Juncosa-Melvin, N;Boivin, GP;Butler, DL
通讯作者：
Butler, DL
影响因子：
1.9
作者：
DUFFY, FJ;SEILER, JG;HERGRUETER, CA
通讯作者：
HERGRUETER, CA