Unraveling Key Metabolomic Alterations in Wheat Embryos Derived from Freshly Harvested and Water-Imbibed Seeds of Two Wheat Cultivars with Contrasting Dormancy Status.
Unraveling Key Metabolomic Alterations in Wheat Embryos Derived from Freshly Harvested and Water-Imbibed Seeds of Two Wheat Cultivars with Contrasting Dormancy Status.
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DOI：
10.3389/fpls.2017.01203
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发表时间：
2017
影响因子：
5.6
通讯作者：
Rohila JS
中科院分区：
文献类型：
--
作者：
Das A;Kim DW;Khadka P;Rakwal R;Rohila JS
小麦收获季节的不适时降雨会引起穗发芽，从而影响小麦的产量和品质。胚的代谢稳态在种子休眠中起作用，决定了成熟谷粒的状态是休眠的（耐PHS的）还是非休眠的（易受PHS的）。很少有人知道直接测量全球代谢物在休眠和非休眠小麦种子的胚胎组织。在这项研究中，生理成熟和新鲜收获的小麦种子的PHS-tolerant（CV。Sukang，休眠）和PHS敏感（cv.白中（Baegjoong，非休眠）品种吸水，并对分离的胚进行高通量、全球非靶向代谢组学分析。Sukang和Baegjoong胚胎在0和48 h吸胀后的代谢产物的仔细比较显示，几个关键的代谢途径[如：脂质，脂肪酸，草酸，激素，棉子糖家族的低聚糖（RFO），和氨基酸]和植物化学物质之间的差异调节休眠和非休眠品种。与Sukang相比，Baegjoong中的大多数膜脂高度降低，这表明细胞膜对吸胀的不稳定性也可能是非休眠小麦品种过早发芽的关键因素。这项研究揭示了几种关键的标志代谢物（例如，RFO：葡萄糖，果糖，麦芽糖和毛蕊花糖），高表达白重吸胀后。此外，数据表明，关键的次生代谢产物和植物化学物质（牡荆素，chrysoeriol，阿魏酸，红景天苷和龙胆酸），与已知的抗氧化性能，在基础水平比较低，在PHS敏感，非休眠品种，白中。总之，这项研究的结果表明，吸胀后，休眠小麦的代谢动态平衡的影响显着小于非休眠小麦。结合蛋白质组学和转录组学研究，本研究的推论将推进对PHS过程中的通路和酶调控的分子理解。
Untimely rains in wheat fields during harvest season can cause pre-harvest sprouting (PHS), which deteriorates the yield and quality of wheat crop. Metabolic homeostasis of the embryo plays a role in seed dormancy, determining the status of the maturing grains either as dormant (PHS-tolerant) or non-dormant (PHS-susceptible). Very little is known for direct measurements of global metabolites in embryonic tissues of dormant and non-dormant wheat seeds. In this study, physiologically matured and freshly harvested wheat seeds of PHS-tolerant (cv. Sukang, dormant) and PHS-susceptible (cv. Baegjoong, non-dormant) cultivars were water-imbibed, and the isolated embryos were subjected to high-throughput, global non-targeted metabolomic profiling. A careful comparison of identified metabolites between Sukang and Baegjoong embryos at 0 and 48 h after imbibition revealed that several key metabolic pathways [such as: lipids, fatty acids, oxalate, hormones, the raffinose family of oligosaccharides (RFOs), and amino acids] and phytochemicals were differentially regulated between dormant and non-dormant varieties. Most of the membrane lipids were highly reduced in Baegjoong compared to Sukang, which indicates that the cell membrane instability in response to imbibition could also be a key factor in non-dormant wheat varieties for their untimely germination. This study revealed that several key marker metabolites (e.g., RFOs: glucose, fructose, maltose, and verbascose), were highly expressed in Baegjoong after imbibition. Furthermore, the data showed that the key secondary metabolites and phytochemicals (vitexin, chrysoeriol, ferulate, salidroside and gentisic acid), with known antioxidant properties, were comparatively low at basal levels in PHS-susceptible, non-dormant cultivar, Baegjoong. In conclusion, the results of this investigation revealed that after imbibition the metabolic homeostasis of dormant wheat is significantly less affected compared to non-dormant wheat. The inferences from this study combined with proteomic and transcriptomic studies will advance the molecular understanding of the pathways and enzyme regulations during PHS.
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DOI：
10.5935/1806-6690.20150034
发表时间：
2015-09-01
期刊：
Revista Ciência Agronômica
影响因子：
--
作者：
Carvalho, Paulo de Tarso;Beleia, Adelaide Del Pino
通讯作者：
Beleia, Adelaide Del Pino
影响因子：
2.4
作者：
Burke, Matthew;Small, Darryl M.;Jones, Oliver A. H.
通讯作者：
Jones, Oliver A. H.
影响因子：
46.9
作者：
Fiehn, O;Kopka, J;Willmitzer, L
通讯作者：
Willmitzer, L
影响因子：
7.2
作者：
Carrera, Esther;Holman, Tara;Holdsworth, Michael J.
通讯作者：
Holdsworth, Michael J.
影响因子：
7.2
作者：
Caliskan, M;Cuming, AC
通讯作者：
Cuming, AC