Two actin-interacting protein 1 isoforms function redundantly in the somatic gonad and are essential for reproduction in Caenorhabditis elegans.
Two actin-interacting protein 1 isoforms function redundantly in the somatic gonad and are essential for reproduction in Caenorhabditis elegans.
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DOI：
10.1002/cm.21152
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发表时间：
2014-01
期刊：
影响因子：
2.9
通讯作者：
Ono, Shoichiro
中科院分区：
文献类型：
--
作者：
Ono, Kanako;Ono, Shoichiro
关键词：
秀丽隐杆线虫的体细胞性腺在排卵期间表现出高度调节的收缩性，这是成功繁殖所必需的。近端卵巢肌上皮鞘中的非横纹肌动蛋白丝网络提供收缩力以推动成熟卵母细胞排卵，但对收缩肌动蛋白网络的组装和调节机制知之甚少。在这里，我们表明，肌动蛋白相互作用蛋白1（AIP 1）是必不可少的收缩肌动蛋白网络的组装在肌上皮鞘。AIP 1在肌动蛋白解聚因子（ADF）/cofilin的存在下促进肌动蛋白丝的分解。C.线虫有两个AIP 1基因，unc-78和aipl-1。单个AIP 1亚型的突变或RNA干扰仅对生殖产生轻微影响。然而，两种AIP 1同种型的同时消耗导致不育。AIP 1缺失的动物显示肌上皮鞘的收缩性非常弱，并且不能排卵成熟的卵母细胞，这导致卵巢内有丝分裂卵母细胞的积累。AIP 1的缺失阻止肌动蛋白网络的组装，并导致肌动蛋白以及ADF/cofilin在肌上皮鞘中的异常聚集。这些结果表明，两种AIP 1亚型在C. elegans生殖
The somatic gonad of the nematode Caenorhabditis elegans exhibits highly regulated contractility during ovulation, which is essential for successful reproduction. Non-striated actin filament networks in the myoepithelial sheath at the proximal ovary provide contractile forces to push a mature oocyte for ovulation, but the mechanism of assembly and regulation of the contractile actin networks is poorly understood. Here, we show that actin-interacting protein 1 (AIP1) is essential for the assembly of the contractile actin networks in the myoepithelial sheath. AIP1 promotes disassembly of actin filaments in the presence of actin depolymerizing factor (ADF)/cofilin. C. elegans has two AIP1 genes, unc-78 and aipl-1. Mutation or RNA interference of a single AIP1 isoform causes only minor impacts on reproduction. However, simultaneous depletion of the two AIP1 isoforms causes sterility. AIP1-depleted animals show very weak contractility of the myoepithelial sheath and fail to ovulate a mature oocyte, which results in accumulation of endomitotic oocytes in the ovary. Depletion of AIP1 prevents assembly of actin networks and causes abnormal aggregation of actin as well as ADF/cofilin in the myoepithelial sheath. These results indicate that two AIP1 isoforms have redundant roles in assembly of the contractile apparatuses necessary for C. elegans reproduction.
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影响因子：
7.8
作者：
Brieher, William M;Kueh, Hao Yuan;Ballif, Bryan A;Mitchison, Timothy J
通讯作者：
Mitchison, Timothy J
影响因子：
4.5
作者：
Kovacevic I;Orozco JM;Cram EJ
通讯作者：
Cram EJ
DOI：
10.1083/jcb.134.3.699
发表时间：
1996-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Iwasaki K;McCarter J;Francis R;Schedl T
通讯作者：
Schedl T
DOI：
10.1083/jcb.100.3.904
发表时间：
1985-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Epstein HF;Miller DM 3rd;Ortiz I;Berliner GC
通讯作者：
Berliner GC
影响因子：
64.5
作者：
MILLER, DM;ORTIZ, I;EPSTEIN, HF
通讯作者：
EPSTEIN, HF