Nobiletin Inhibits Inflammatory Reaction in Interleukin-1β-Stimulated Human Periodontal Ligament Cells.
Nobiletin Inhibits Inflammatory Reaction in Interleukin-1β-Stimulated Human Periodontal Ligament Cells.
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Nobiletin抑制白介素-1β刺激的人牙周韧带细胞中的炎症反应。
DOI：
10.3390/pharmaceutics13050667
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发表时间：
2021-05-07
期刊：
影响因子：
5.4
通讯作者：
Matsuo T
中科院分区：
文献类型：
--
作者：
Hosokawa Y;Hosokawa I;Ozaki K;Matsuo T
牙周病损中的免疫反应参与了牙周病的进展。因此，寻找一种对牙周病变具有抗炎作用的生物活性物质是非常重要的。本研究旨在检测柑橘果皮中的陈皮素是否能抑制白细胞介素（IL）-1β刺激的人牙周膜细胞（HPDLCs）的炎症反应。通过ELISA评估细胞因子（IL-6、IL-8、CXCL 10、CCL 20和CCL 2）和基质金属蛋白酶（MMP-1和MMP-3）的释放。Western blot检测细胞粘附分子（ICAM-1和VCAM-1）的表达及信号转导通路（核因子（NF）-κB、丝裂原活化蛋白激酶（MAPK）和蛋白激酶B（Akt））的激活。此外，我们发现，诺贝尔碱处理可以抑制NF-κB，MAPK和Akt途径的激活。这些结果表明，川陈皮素可通过抑制多种信号转导途径，包括NF-κB、MAPKs和Akt，抑制IL-1β刺激的HPDLCs的炎症反应。
The immune response in periodontal lesions is involved in the progression of periodontal disease. Therefore, it is important to find a bioactive substance that has anti-inflammatory effects in periodontal lesions. This study aimed to examine if nobiletin, which is found in the peel of citrus fruits, could inhibit inflammatory responses in interleukin (IL)-1β-stimulated human periodontal ligament cells (HPDLCs). The release of cytokines (IL-6, IL-8, CXCL10, CCL20, and CCL2) and matrix metalloproteinases (MMP-1 and MMP-3) was assessed by ELISA. The expression of cell adhesion molecules (ICAM-1and VCAM-1) and the activation of signal transduction pathways (nuclear factor (NF)-κB, mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt)) in HPDLCs were detected by Western blot analysis. Our experiments revealed that nobiletin decreased the expression of inflammatory cytokines, cell adhesion molecules, and MMPs in IL-1β-stimulated HPDLCs. Moreover, we revealed that nobiletin treatment could suppress the activation of the NF-κB, MAPKs, and Akt pathways. These findings indicate that nobiletin could inhibit inflammatory reactions in IL-1β-stimulated HPDLCs by inhibiting multiple signal transduction pathways, including NF-κB, MAPKs, and Akt.
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影响因子：
7.3
作者：
Sahingur SE;Yeudall WA
通讯作者：
Yeudall WA
DOI：
10.1155/2016/2918796
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Huang H;Li L;Shi W;Liu H;Yang J;Yuan X;Wu L
通讯作者：
Wu L
DOI：
10.1590/1678-775720140259
发表时间：
2015-05
期刊：
Journal of applied oral science : revista FOB
影响因子：
--
作者：
Silva N;Abusleme L;Bravo D;Dutzan N;Garcia-Sesnich J;Vernal R;Hernández M;Gamonal J
通讯作者：
Gamonal J
影响因子：
2.9
作者：
Chen J;Creed A;Chen AY;Huang H;Li Z;Rankin GO;Ye X;Xu G;Chen YC
通讯作者：
Chen YC
影响因子：
5
作者：
Zhou, Cheng-Hua;Wu, Xiao-Hong;Wu, Yu-Qing
通讯作者：
Wu, Yu-Qing