Working together to control mutation: how collective peroxide detoxification determines microbial mutation rate plasticity
Working together to control mutation: how collective peroxide detoxification determines microbial mutation rate plasticity
复制标题
共同努力控制突变：集体过氧化物解毒如何决定微生物突变率可塑性
DOI：
10.1101/2023.09.27.557722
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Green R
中科院分区：
文献类型：
--
作者：
Green R
诱变受多种环境因素的影响。因此，进化不仅在选择方面取决于环境，而且在决定种群中可用的变异方面也取决于环境。在许多微生物物种中，突变率和种群密度之间的反比关系就是这样一种环境依赖性。在这里，我们确定了负责这种突变率可塑性的机制。利用动态计算模型和变异率估计，我们表明突变率和种群密度之间的负相关关系源于微生物种群控制过氧化氢浓度的集体能力。我们证明，当大肠杆菌群体缺乏过氧化氢降解时，这种与密度相关的突变率可塑性就会丧失。我们进一步表明，由于混合群体中存在野生型细胞，较密集群体中突变率的降低在过氧化氢降解缺陷细胞中得以恢复。总之，这些模型指导的实验为密度相关的突变率可塑性提供了一种机制解释，适用于所有生命领域，并将突变率框架为微生物群落组成形成的动态特征。
Mutagenesis is responsive to many environmental factors. Evolution therefore depends on the environment not only for selection but also in determining the variation available in a population. One such environmental dependency is the inverse relationship between mutation rates and population density in many microbial species. Here we determine the mechanism responsible for this mutation rate plasticity. Using dynamical computational modelling andin vivomutation rate estimation we show that the negative relationship between mutation rate and population density arises from the collective ability of microbial populations to control concentrations of hydrogen peroxide. We demonstrate a loss of this density-associated mutation rate plasticity whenEscherichia colipopulations are deficient in the degradation of hydrogen peroxide. We further show that the reduction in mutation rate in denser populations is restored in peroxide degradation-deficient cells by the presence of wild-type cells in a mixed population. Together, these model-guided experiments provide a mechanistic explanation for density-associated mutation rate plasticity, applicable across all domains of life, and frames mutation rate as a dynamic trait shaped by microbial community composition.
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