Phosphoproteome analysis reveals the involvement of protein dephosphorylation in ethylene-induced corolla senescence in petunia.
Phosphoproteome analysis reveals the involvement of protein dephosphorylation in ethylene-induced corolla senescence in petunia.
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磷酸蛋白质组分析揭示蛋白质去磷酸化参与乙烯诱导的矮牵牛花冠衰老
DOI：
10.1186/s12870-021-03286-x
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发表时间：
2021-11-03
影响因子：
5.3
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Zhong S;Sang L;Zhao Z;Deng Y;Liu H;Yu Y;Liu J
背景衰老代表花朵发育的最后阶段。磷酸化是调节蛋白质功能的关键翻译后修饰，在植物生长和发育过程中可能比磷酸酶更需要激酶。然而，人们对花朵衰老过程中整体磷酸化的变化知之甚少。结果在这项工作中，我们定量研究了乙烯或空气处理后的矮牵牛磷酸化蛋白质组。总共鉴定了 1184 个蛋白质组中的 2170 个磷酸位点，其中对 1124 个蛋白质中的 2059 个位点进行了定量。令我们惊讶的是，使用 1.5 倍阈值 (FDR < 0.05)，乙烯处理导致 697 个磷酸位点下调，仅 117 个磷酸位点上调，这表明乙烯负向调节整体磷酸化水平，并且许多蛋白质的磷酸化在花衰老过程中不是必需的。磷酸化蛋白质组分析表明乙烯通过磷酸化水平调节乙烯和ABA信号转导途径。乙烯诱导的去磷酸化的主要靶点之一是植物mRNA剪接机制，乙烯处理增加了矮牵牛花冠中前体RNA选择性剪接事件的数量。结论蛋白质去磷酸化在乙烯诱导的衰老中可能发挥重要作用，乙烯处理增加了矮牵牛花冠中AS前体RNA的数量。
BackgroundSenescence represents the last stage of flower development. Phosphorylation is the key posttranslational modification that regulates protein functions, and kinases may be more required than phosphatases during plant growth and development. However, little is known about global phosphorylation changes during flower senescence.ResultsIn this work, we quantitatively investigated the petunia phosphoproteome following ethylene or air treatment. In total, 2170 phosphosites in 1184 protein groups were identified, among which 2059 sites in 1124 proteins were quantified. To our surprise, treatment with ethylene resulted in 697 downregulated and only 117 upregulated phosphosites using a 1.5-fold threshold (FDR < 0.05), which showed that ethylene negatively regulates global phosphorylation levels and that phosphorylation of many proteins was not necessary during flower senescence. Phosphoproteome analysis showed that ethylene regulates ethylene and ABA signalling transduction pathways via phosphorylation levels. One of the major targets of ethylene-induced dephosphorylation is the plant mRNA splicing machinery, and ethylene treatment increases the number of alternative splicing events of precursor RNAs in petunia corollas.ConclusionsProtein dephosphorylation could play an important role in ethylene-induced senescence, and ethylene treatment increased the number of AS precursor RNAs in petunia corollas.
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影响因子：
8.7
作者：
Du L;Song J;Forney C;Palmer LC;Fillmore S;Zhang Z
通讯作者：
Zhang Z
DOI：
10.1073/pnas.011405198
发表时间：
2001-01-02
影响因子：
11.1
作者：
Frye, CA;Tang, DZ;Innes, RW
通讯作者：
Innes, RW
影响因子：
--
作者：
Chen, Ruiqiang;Binder, Brad M.;Cooper, Bret
通讯作者：
Cooper, Bret
影响因子：
6.9
作者：
Bai S;Willard B;Chapin LJ;Kinter MT;Francis DM;Stead AD;Jones ML
通讯作者：
Jones ML
影响因子：
7.2
作者：
Joo, Sunjoo;Liu, Yidong;Zhang, Shuqun
通讯作者：
Zhang, Shuqun