Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield.
Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield.
复制标题
DOI：
10.1093/jxb/ert370
复制
发表时间：
2014-01
影响因子：
6.9
通讯作者：
Simmons CR
中科院分区：
文献类型：
--
作者：
Guo M;Rupe MA;Wei J;Winkler C;Goncalves-Butruille M;Weers BP;Cerwick SF;Dieter JA;Duncan KE;Howard RJ;Hou Z;Löffler CM;Cooper M;Simmons CR
单个转基因ARGOS1对田间生长的杂交玉米的产量有积极影响。来自优良杂交育种种质的两个主要等位基因在转基因功效上不同，但两个等位基因组合在转基因堆叠中优于各自单独，与单位点杂种优势效应一致。由于这些性状的复杂遗传性质和环境依赖性，作物产量和耐旱性的改良具有挑战性。本研究报告，转基因玉米ARGOS1（ZAR 1）的过度表达增强玉米器官生长，籽粒产量和干旱胁迫耐受性。ZAR 1转基因表现出环境互作，在温带干旱环境下产量增加，在温带潮湿或高纬度环境下产量减少。天然ZAR 1等位基因变异与干旱胁迫耐受性相关两个创始人等位基因中确定的中期成熟种质的北美现在占主导地位的先锋的现代育种计划，并有不同的蛋白质，启动子和表达模式。这两个主要等位基因表现出杂种优势群划分，其中一个在先锋的女性和男性杂种优势群中占主导地位，分别。这两个等位基因在杂合时也与有利的作物表现相关。等位基因特异性转基因测试表明，在这里讨论的两个等位基因中，每个等位基因对产量和环境相互作用的影响不同。此外，当转基因堆叠在一起的等位基因对表现出产量和环境性能的优势，任何一个单一的等位基因，类似的杂种优势效应。这项工作证明了天然等位基因的转基因功效的差异，这些差异反映了它们与杂交育种性能的关联。
A single transgene ARGOS1 positively impacts yield of field-grown hybrid maize. Two predominant alleles from elite hybrid breeding germplasm differed in transgene efficacy, but both alleles combined in a transgenic stack outperformed each alone, consistent with a single-locus heterotic effect Crop improvement for yield and drought tolerance is challenging due to the complex genetic nature of these traits and environmental dependencies. This study reports that transgenic over-expression of Zea mays ARGOS1 (ZAR1) enhanced maize organ growth, grain yield, and drought-stress tolerance. The ZAR1 transgene exhibited environmental interactions, with yield increase under Temperate Dry and yield reduction under Temperate Humid or High Latitude environments. Native ZAR1 allele variation associated with drought-stress tolerance. Two founder alleles identified in the mid-maturity germplasm of North America now predominate in Pioneer’s modern breeding programme, and have distinct proteins, promoters and expression patterns. These two major alleles show heterotic group partitioning, with one predominant in Pioneer’s female and the other in the male heterotic groups, respectively. These two alleles also associate with favourable crop performance when heterozygous. Allele-specific transgene testing showed that, of the two alleles discussed here, each allele differed in their impact on yield and environmental interactions. Moreover, when transgenically stacked together the allelic pair showed yield and environmental performance advantages over either single allele, resembling heterosis effects. This work demonstrates differences in transgenic efficacy of native alleles and the differences reflect their association with hybrid breeding performance.
登录
查看更多内容
影响因子：
5.4
作者：
Guo, Mei;Rupe, Mary A.;Bowen, Ben
通讯作者：
Bowen, Ben
影响因子：
3.3
作者：
Boer, Martin P.;Wright, Deanne;van Eeuwijk, Fred A.
通讯作者：
van Eeuwijk, Fred A.
影响因子：
2.3
作者：
Hammer, Graeme L.;Dong, Zhanshan;Cooper, Mark
通讯作者：
Cooper, Mark
影响因子：
56.9
作者：
HAYASHI, H;CZAJA, I;WALDEN, R
通讯作者：
WALDEN, R
影响因子：
6.1
作者：
Duke, Stephen O.
通讯作者：
Duke, Stephen O.