The core genome of the anaerobic oral pathogenic bacterium Porphyromonas gingivalis.
The core genome of the anaerobic oral pathogenic bacterium Porphyromonas gingivalis.
复制标题
DOI：
10.1186/1471-2180-10-252
复制
发表时间：
2010-09-29
期刊：
影响因子：
4.2
通讯作者：
Crielaard W
中科院分区：
文献类型：
--
作者：
Brunner J;Wittink FR;Jonker MJ;de Jong M;Breit TM;Laine ML;de Soet JJ;Crielaard W
革兰氏阴性厌氧菌牙龈卟啉单胞菌长期以来被认为是牙周炎的病原体。牙周炎是牙齿支持组织的慢性感染性疾病，最终导致牙齿丧失。牙龈卟啉单胞菌的荚膜多糖（CPS）是其重要的毒力决定因子。已经描述了七种荚膜血清型。在这里，我们使用基于微阵列的比较基因组杂交分析（CGH）来分析每种荚膜血清型的代表和针对高毒力和测序的W83菌株的非包囊化菌株。我们使用拟南芥阴性对照探针定义了缺失呼叫，目的是区分由于突变和基因获得/丢失引起的畸变。我们的分析使我们能够在每个测试菌株中调用异常基因，缺失基因和分歧区域。描述了一个保守的核心牙龈卟啉单胞菌基因组，其由来自测序的W83菌株的80%的分析基因组成。试验菌株与对照菌株W83之间的基因变异率为8.2%~ 13.7%。在异常基因中发现了许多CPS生物合成基因，大多数其他毒力相关基因都存在于保守的核心基因组中。高毒力菌株与低毒力菌株的比较表明，hmuS，一个假定的CobN/镁螯合酶参与血红素摄取，可能是一个更相关的毒力决定因素比以前预期的。此外，对39个W83特异性基因的描述可以更深入地了解为什么该菌株比其他菌株更具毒性。对牙龈卟啉单胞菌荚膜血清型的遗传内容的分析允许描述牙龈卟啉单胞菌核心基因组。来自三个重复的杂交实验的三种类型的分析的高分辨率数据可以解释牙龈卟啉单胞菌菌株之间比以前认识到的更高的分歧。
The Gram negative anaerobic bacterium Porphyromonas gingivalis has long been recognized as a causative agent of periodontitis. Periodontitis is a chronic infectious disease of the tooth supporting tissues eventually leading to tooth-loss. Capsular polysaccharide (CPS) of P. gingivalis has been shown to be an important virulence determinant. Seven capsular serotypes have been described. Here, we used micro-array based comparative genomic hybridization analysis (CGH) to analyze a representative of each of the capsular serotypes and a non-encapsulated strain against the highly virulent and sequenced W83 strain. We defined absent calls using Arabidopsis thaliana negative control probes, with the aim to distinguish between aberrations due to mutations and gene gain/loss. Our analyses allowed us to call aberrant genes, absent genes and divergent regions in each of the test strains. A conserved core P. gingivalis genome was described, which consists of 80% of the analyzed genes from the sequenced W83 strain. The percentage of aberrant genes between the test strains and control strain W83 was 8.2% to 13.7%. Among the aberrant genes many CPS biosynthesis genes were found. Most other virulence related genes could be found in the conserved core genome. Comparing highly virulent strains with less virulent strains indicates that hmuS, a putative CobN/Mg chelatase involved in heme uptake, may be a more relevant virulence determinant than previously expected. Furthermore, the description of the 39 W83-specific genes could give more insight in why this strain is more virulent than others. Analyses of the genetic content of the P. gingivalis capsular serotypes allowed the description of a P. gingivalis core genome. The high resolution data from three types of analysis of triplicate hybridization experiments may explain the higher divergence between P. gingivalis strains than previously recognized.
登录
查看更多内容
影响因子：
6.7
作者：
Hugoson, Anders;Sjodin, Bengt;Norderyd, Ola
通讯作者：
Norderyd, Ola
影响因子：
12.3
作者：
Dufresne, Alexis;Ostrowski, Martin;Scanlan, David J.;Garczarek, Laurence;Mazard, Sophie;Palenik, Brian P.;Paulsen, Ian T.;de Marsac, Nicole Tandeau;Wincker, Patrick;Dossat, Carole;Ferriera, Steve;Johnson, Justin;Post, Anton F.;Hess, Wolfgang R.;Partensky, Frederic
通讯作者：
Partensky, Frederic
影响因子：
3.1
作者：
Hosogi, Y;Duncan, MJ
通讯作者：
Duncan, MJ
影响因子：
3.2
作者：
Chen, T;Hosogi, Y;Duncan, MJ
通讯作者：
Duncan, MJ
影响因子：
--
作者：
Laine, ML;van Winkelhoff, AJ
通讯作者：
van Winkelhoff, AJ