Outer membrane vesicles as a candidate vaccine against edwardsiellosis.
Outer membrane vesicles as a candidate vaccine against edwardsiellosis.
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DOI：
10.1371/journal.pone.0017629
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发表时间：
2011-03-08
期刊：
影响因子：
3.7
通讯作者：
Jung TS
中科院分区：
文献类型：
--
作者：
Park SB;Jang HB;Nho SW;Cha IS;Hikima J;Ohtani M;Aoki T;Jung TS
感染迟缓爱德华氏菌是一种革兰氏阴性细菌，导致海水和淡水鱼的高发病率和死亡率。革兰氏阴性菌释放的外膜小泡(OMV)在细菌致病和宿主免疫应答中发挥重要作用，但迟发性埃希氏菌OMV的这种作用尚未被描述。在本研究中，我们研究了OMVS的蛋白质组组成和OMVS在自然宿主中的免疫刺激作用，以及OMVS作为疫苗用于预防迟发性艾美耳球虫感染的效果。在OMV中共鉴定出74种蛋白质，来自不同的亚细胞组分。其中包括多种重要的毒力因子，如溶血素、OmpA、孔蛋白、GAPDH、EseB、ESEC、EseD、EvpC、EvpP、脂蛋白、鞭毛蛋白和菌毛蛋白。与注射福尔马林灭活的迟发性E.tarda相比，当OMV被注射到橄榄牙鲆体内时，观察到显著诱导编码IL-1β、IL-6、肿瘤坏死因子α和干扰素γ的mRNAs。在疫苗试验中，给予OMV的比目鱼比对照鱼得到了更有效的保护(p<；0.0001)。对OMV的研究不仅有助于了解迟缓艾美耳球虫的发病机制，而且有助于研制有效的爱德华氏菌病疫苗。
Infection with Edwardsiella tarda, a Gram-negative bacterium, causes high morbidity and mortality in both marine and freshwater fish. Outer membrane vesicles (OMVs) released from Gram-negative bacteria are known to play important roles in bacterial pathogenesis and host immune responses, but no such roles for E. tarda OMVs have yet been described. In the present study, we investigated the proteomic composition of OMVs and the immunostimulatory effect of OMVs in a natural host, as well as the efficacy of OMVs when used as a vaccine against E. tarda infection. A total of 74 proteins, from diverse subcellular fractions, were identified in OMVs. These included a variety of important virulence factors, such as hemolysin, OmpA, porin, GAPDH, EseB, EseC, EseD, EvpC, EvpP, lipoprotein, flagellin, and fimbrial protein. When OMVs were administrated to olive flounder, significant induction of mRNAs encoding IL-1β, IL-6, TNFα, and IFNγ was observed, compared with the levels seen in fish injected with formalin-killed E. tarda. In a vaccine trial, olive flounder given OMVs were more effectively protected (p<0.0001) than were control fish. Investigation of OMVs may be useful not only for understanding the pathogenesis of E. tarda but also in development of an effective vaccine against edwardsiellosis.
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影响因子：
4.7
作者：
Castro, N.;Toranzo, A. E.;Magarinos, B.
通讯作者：
Magarinos, B.
DOI：
10.1016/0005-2736(76)90058-4
发表时间：
1976-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
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WITHOLT, B
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
Sengupta, U.