Development of a nanoLC LTQ orbitrap mass spectrometric method for profiling glycans derived from plasma from healthy, benign tumor control, and epithelial ovarian cancer patients.
Development of a nanoLC LTQ orbitrap mass spectrometric method for profiling glycans derived from plasma from healthy, benign tumor control, and epithelial ovarian cancer patients.
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DOI：
10.1021/ac802262w
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发表时间：
2009-02-01
影响因子：
7.4
通讯作者：
Muddiman, David C.
中科院分区：
文献类型：
--
作者：
Bereman, Michael S.;Williams, Taufika Islam;Muddiman, David C.
我们报告了无分流纳米流液相色谱质谱分析的发展，从血浆中的糖蛋白化学裂解的聚糖。多孔石墨化的碳在反相条件下操作和基于酰胺的固定相在亲水相互作用条件下操作的聚糖分离进行定量比较。两种固定相表现出相似的柱效率和优异的保留时间重现性，无需内标来校正保留时间偏移。在5天分析期间，两种固定相的平均保留时间的95%置信区间均为±4秒;然而，观察到酰胺固定相更耐用。小于2 ppm的高质量测量准确度和裂解光谱提供了高度可信的鉴别沿着结构信息。此外，数据进行了比较之间的样品来自10个健康对照，10个对照与良性妇科肿瘤的鉴别诊断，和10个患病的上皮性卵巢癌患者（EOC）。发现两种岩藻糖基化聚糖在健康对照中上调，并提供了准确的诊断价值，受试者工作特征曲线下面积为0.87。然而，当用于区分EOC与良性肿瘤对照样品时，这些相同的聚糖提供了显著较低的诊断价值，曲线下面积为0.73。
We report the development of split-less nano-flow liquid chromatography mass spectrometric analysis of glycans chemically cleaved from glycoproteins in plasma. Porous graphitized carbon operating under reverse-phase conditions and an amide-based stationary phase operating under hydrophilic interaction conditions are quantitatively compared for glycan separation. Both stationary phases demonstrated similar column efficiencies and excellent retention time reproducibility without an internal standard to correct for retention time shift. The 95% confidence intervals of the mean retention times were ±4 seconds across 5 days of analysis for both stationary phases; however, the amide stationary phase was observed to be more robust. The high mass measurement accuracy of less than 2 ppm and fragmentation spectra provided highly confident identifications along with structural information. In addition, data are compared amongst samples derived from 10 healthy controls, 10 controls with a differential diagnosis of benign gynecologic tumors, and 10 diseased epithelial ovarian cancer patients (EOC). Two fucosylated glycans were found to be up-regulated in healthy controls and provided an accurate diagnostic value with an area under the receiver operator characteristic curve of 0.87. However, these same glycans provided a significantly less diagnostic value when used to differentiate EOC from benign tumor control samples with an area under the curve of 0.73.
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DOI：
10.1016/s0021-9673(00)96972-3
发表时间：
1990-01-19
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALPERT, AJ
通讯作者：
ALPERT, AJ
影响因子：
7.4
作者：
FOLEY, JP;DORSEY, JG
通讯作者：
DORSEY, JG
影响因子：
7.4
作者：
Bahr, U;Pfenninger, A;Stahl, B
通讯作者：
Stahl, B
影响因子：
7
作者：
Kirmiz, Crystal;Li, Bensheng;Miyamoto, Suzanne
通讯作者：
Miyamoto, Suzanne
影响因子：
4
作者：
Casey, RC;Oegema, TR;Skubitz, APN
通讯作者：
Skubitz, APN