Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging.
Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging.
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DOI：
10.1021/acsnano.5b03430
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发表时间：
2015-09-22
期刊：
影响因子：
17.1
通讯作者：
Landes, Christy F.
中科院分区：
文献类型：
--
作者：
Kisley, Lydia;Brunetti, Rachel;Tauzin, Lawrence J.;Shuang, Bo;Yi, Xiyu;Kirkeminde, Alec W.;Higgins, Daniel A.;Weiss, Shimon;Landes, Christy F.
多孔材料如细胞胞质溶胶、水凝胶和嵌段共聚物具有决定宏观尺度性质的纳米尺度特征。由于成像、样品制备和计算挑战，用当前技术表征纳米孔的结构是困难的。我们产生了一个超分辨率的光学图像，同时表征纳米尺寸和扩散动力学多孔结构内的相关随机波动从扩散荧光探针在样品的孔，在这里被称为“荧光相关光谱超分辨率光学波动成像”或“fcsSOFI”。模拟结果表明，结构特征和扩散性能可以准确地获得亚衍射极限分辨率。我们应用我们的技术来图像琼脂糖水凝胶和水性溶致液晶凝胶。的非均质孔分辨率提高了2倍，通过我们的方法相比，衍射限制的荧光成像和单粒子跟踪和扩散系数准确地获得。此外，fcsSOFI允许多孔材料的快速和高通量表征。fcsSOFI可应用于软多孔环境，例如水凝胶、聚合物和膜，以及硬材料，例如沸石和中孔二氧化硅。
Porous materials such as cellular cytosol, hydrogels, and block copolymers have nanoscale features that determine macroscale properties. Characterizing the structure of nanopores is difficult with current techniques due to imaging, sample preparation, and computational challenges. We produce a super-resolution optical image that simultaneously characterizes the nanometer dimensions of and diffusion dynamics within porous structures by correlating stochastic fluctuations from diffusing fluorescent probes in the pores of the sample, dubbed here as “fluorescence correlation spectroscopy super-resolution optical fluctuation imaging” or “fcsSOFI”. Simulations demonstrate that structural features and diffusion properties can be accurately obtained at sub-diffraction-limited resolution. We apply our technique to image agarose hydrogels and aqueous lyotropic liquid crystal gels. The heterogeneous pore resolution is improved by up to a factor of 2, and diffusion coefficients are accurately obtained through our method compared to diffraction-limited fluorescence imaging and single-particle tracking. Moreover, fcsSOFI allows for rapid and high-throughput characterization of porous materials. fcsSOFI could be applied to soft porous environments such hydrogels, polymers, and membranes in addition to hard materials such as zeolites and mesoporous silica.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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通讯作者：
Landes, Christy F.
DOI：
10.1073/pnas.0907866106
发表时间：
2009-12-29
影响因子：
11.1
作者：
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通讯作者：
Enderlein, J.
DOI：
10.1073/pnas.1204917109
发表时间：
2012-07-03
影响因子：
11.1
作者：
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通讯作者：
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