Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents.
Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents.
复制标题
DOI：
10.1002/jmr.2206
复制
发表时间：
2012-08
影响因子：
2.7
通讯作者：
Landes, Christy F.
中科院分区：
文献类型：
--
作者：
Daniels, Charlisa R.;Kisley, Lydia;Kim, Hannah;Chen, Wen-Hsiang;Poongavanam, Mohan-Vivekanandan;Reznik, Carmen;Kourentzi, Katerina;Willson, Richard C.;Landes, Christy F.
关键词：
离子交换层析（IEX）依赖于吸附剂和被吸附物之间的静电相互作用，并广泛用于蛋白质纯化。传统的IEX利用单电荷和随机分散在吸附剂上的配体，产生潜在吸附位点的非均匀分布。与总电荷密度相同的分散电荷离子交换剂相比，分散电荷离子交换剂表现出更高的亲和力、容量和选择性。在目前的工作中，我们监测的运输行为的阴离子蛋白质附近的簇电荷吸附剂表面使用荧光相关光谱。我们可以解决蛋白质的自由扩散，受阻扩散和关联裸玻璃，琼脂糖涂层，琼脂糖聚集肽表面，表明该方法可用于了解并最终优化聚集电荷吸附剂和其他表面相互作用在分子尺度上。
Ion-exchange chromatography (IEX) relies on electrostatic interactions between the adsorbent and the adsorbate, and is used extensively in protein purification. Conventional IEX utilizes ligands that are singly charged and randomly dispersed over the adsorbent, creating a heterogeneous distribution of potential adsorption sites. Clustered-charge ion exchangers exhibit higher affinity, capacity, and selectivity than their dispersed-charge counterparts of the same total charge density. In the present work, we monitored the transport behavior of an anionic protein near clustered-charge adsorbent surfaces using Fluorescence Correlation Spectroscopy. We can resolve protein free diffusion, hindered diffusion and association with bare glass, agarose-coated, and agarose-clustered peptide surfaces, demonstrating that this method can be used to understand and ultimately optimize clustered charge adsorbent and other surface interactions at the molecular scale.
登录
查看更多内容
DOI：
10.1021/la904749z
发表时间：
2010-04-06
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Daniels CR;Reznik C;Landes CF
通讯作者：
Landes CF
影响因子：
15
作者：
BOYD, GE;ADAMSON, AW;MYERS, LS
通讯作者：
MYERS, LS
影响因子：
3.9
作者：
Ajikumar, Parayil Kumaran;Kiat, Jin;Too, Heng-Phon
通讯作者：
Too, Heng-Phon
影响因子：
5.8
作者：
Daniels, Charlisa R.;Reznik, Carmen;Landes, Christy F.
通讯作者：
Landes, Christy F.
影响因子：
3.2
作者：
Panchuk-Voloshina, N;Haugland, RP;Haugland, RP
通讯作者：
Haugland, RP