Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
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高多孔性壳聚糖基骨类似物中组织特异性间充质干细胞依赖性成骨。
DOI：
10.1002/sctm.19-0385
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发表时间：
2021-03
影响因子：
6
通讯作者：
Mohanty S
中科院分区：
文献类型：
--
作者：
Midha S;Jain KG;Bhaskar N;Kaur A;Rawat S;Giri S;Basu B;Mohanty S
在传统的制造技术中，冷冻干燥工艺已被广泛研究用于聚合物植入物。然而，尚未探索对干细胞祖细胞依赖性细胞功能调节的理解和高度多孔支架的早期骨整合的定量分析。在这里，我们开发了一种新型的，高度多孔，多材料复合材料，壳聚糖/羟基磷灰石/聚己内酯（CHT/HA/PCL）。使用来自三种组织来源的间充质干细胞（MSC）进行了体外研究：人骨髓来源的MSC（BM-MSC）、脂肪来源的MSC（AD-MSC）和沃顿氏胶质来源的MSC（WJ-MSC）。虽然细胞附着和代谢活性[3 - 4，5-二甲基噻唑-2-基-（2，5二苯基-2H-四唑溴化物）测定]在WJ-MSC-负载的CHT/HA/PCL复合材料、扫描电子显微镜、真实的-时间基因表达中得到进一步增强（碱性磷酸酶[ALP]、I型胶原[Col I]、骨钙素[OCN]和骨形态发生蛋白4 [BMP-4]）和免疫染色（COL I、β-连环蛋白、OCN和硬化蛋白[SOST]）仅在载有BM-MSC-的CHT/HA/PCL复合材料上显示出明显的骨生成和终末分化。CHT/HA/PCL复合材料上增强的细胞功能性被解释为表面性质和MSC的最佳来源之间的相互作用。此外，在大鼠胫骨模型中，6周以上的骨生成证实，与仅支架组和仅缺损组相比，BM-MSC负载复合材料的骨体积与总体积的比率更好。3D多孔结构（孔径范围为20 - 200 μ m）的临床符合性组合，以及受控的体外降解和早期骨整合，确立了CHT/HA/PCL复合材料作为潜在松质骨类似物的潜力。使用冷冻干燥方法开发了基于壳聚糖的多材料。使用三种临床相关的常用人干细胞（骨髓[BM]-、脂肪-、胎儿来源的MSC）进行骨整合，评估生物相容性。在体外和体内记录了BM-MSC-loaded复合物用于重建临床前大鼠胫骨缺损的更好的骨整合。
Among conventional fabrication techniques, freeze‐drying process has widely been investigated for polymeric implants. However, the understanding of the stem cell progenitor‐dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored. Here, we developed a novel, highly porous, multimaterial composite, chitosan/hydroxyapatite/polycaprolactone (CHT/HA/PCL). The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow‐derived MSCs (BM‐MSCs), adipose‐derived MSCs (AD‐MSCs), and Wharton's jelly‐derived MSCs (WJ‐MSCs). Although cell attachment and metabolic activity [3‐4,5‐dimethylthiazol‐2yl‐(2,5 diphenyl‐2H‐tetrazoliumbromide) assay] were ore enhanced in WJ‐MSC‐laden CHT/HA/PCL composites, scanning electron microscopy, real‐time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP‐4]), and immunostaining (COL I, β‐CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM‐MSC‐laden CHT/HA/PCL composites only. The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs. In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM‐MSC‐laden composites over scaffold‐only and defect‐only groups. The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog. A chitosan‐based, multimaterial was developed using freeze‐drying approach. The biocompatibility was assessed using three popular human stem cells of clinical relevance (bone marrow [BM]‐, adipose‐, fetal‐derived MSCs) was carried out for osseointegration. A better osseointegration was recorded with BM‐MSC‐laden composites in vitro and in vivo for reconstruction of preclinical rat tibial defects.
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影响因子：
7.5
作者：
Batsali AK;Pontikoglou C;Koutroulakis D;Pavlaki KI;Damianaki A;Mavroudi I;Alpantaki K;Kouvidi E;Kontakis G;Papadaki HA
通讯作者：
Papadaki HA
影响因子：
--
作者：
Florencio-Silva R;Sasso GR;Sasso-Cerri E;Simões MJ;Cerri PS
通讯作者：
Cerri PS
影响因子：
5.6
作者：
Kaempfen A;Todorov A;Güven S;Largo RD;Jaquiéry C;Scherberich A;Martin I;Schaefer DJ
通讯作者：
Schaefer DJ
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
4
作者：
Hsieh, Jui-Yu;Fu, Yu-Show;Wang, Hsei-Wei
通讯作者：
Wang, Hsei-Wei