(17)O NMR spectroscopy-assisted in vitro bioactivity studies of the intermediates formed via Na(2)S and RSNO cross-linking reactions.
(17)O NMR spectroscopy-assisted in vitro bioactivity studies of the intermediates formed via Na(2)S and RSNO cross-linking reactions.
复制标题
17O NMR 波谱辅助 Na2S 和 RSNO 交联反应形成的中间体的体外生物活性研究
作者：
硫化物和S-亚硝基硫醇部分之间的交联反应已被深入研究，亚硝酸盐/亚硝酸（SNO−/HSNO）以及亚硝基过硫化物（SSNO−）被报道为可作为一氧化氮（NO）储存库的中间体。然而，关于SNO−/HSNO和SSNO−的稳定性和生物活性仍然存在争议。为了研究SNO−和SSNO−的化学性质和生物活性，我们开始使用UV-Vis和15 N NMR光谱技术以及新的17 O NMR方法重新表征反应中间体。通过细胞培养实验评估了SNO−和SSNO−对细胞NO和cGMP水平的影响，并使用人脐静脉内皮细胞（HUVEC）评估了SNO−和SSNO−对细胞增殖、迁移和毛细血管样结构形成的影响。通过这项工作，在各种制备条件下阐明了SNO−和SSNO−的特征峰和半衰期。生物学试验表明，SSNO-增加了细胞NO和cGMP水平，还促进了细胞增殖，迁移和刺激血管生成，而相反，SNO-没有表现出这些作用。通过UV-Vis、15 N NMR和17 O NMR光谱技术，我们表征了RSNO和Na 2S交联反应的中间体（SSNO-和SNO-）。我们发现SSNO-在细胞培养实验中可以作为NO库。
The cross-linking reaction between sulfide and S-nitrosothiol moieties has been intensively investigated and thionitrite/thionitrous acid (SNO−/HSNO) as well as nitrosopersulfide (SSNO−) were reported to be the intermediates that could serve as reservoirs for nitric oxide (NO). However, debate still exists regarding the stability and biological activity of SNO−/HSNO and SSNO−. In order to investigate the chemical properties and biological activity of SNO− and SSNO−, we set out to re-characterize the reaction intermediates using UV-Vis and 15N NMR spectroscopy techniques, as well as a new 17O NMR approach. The effects of SNO− and SSNO− on cellular NO and cGMP levels were assessed via cell culture experiments, and also the effects of SNO− and SSNO− on cell proliferation, migration, and capillary-like structure formation were evaluated with human umbilical vein endothelial cells (HUVEC). Through this work, the characteristic peaks and half-lives of SNO− and SSNO− were elucidated under various preparation conditions. The biological assays demonstrated that SSNO− increased the cellular NO and cGMP levels and also facilitated cell proliferation, migration and stimulated angiogenesis, while in contrast SNO− did not exhibit these effects. By using UV-Vis, 15N NMR and 17O NMR spectroscopy techniques, we characterized the intermediates (SSNO− and SNO−) obtained from RSNO and Na2S cross-linking reaction. We found that SSNO− could serve as NO reservoir in cell culture experiments.
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影响因子：
15
作者：
Filipovic, Milos R.;Miljkovic, Jan Lj;Nauser, Thomas;Royzen, Maksim;Klos, Katharina;Shubina, Tatyana;Koppenol, Willem H.;Lippard, Stephen J.;Ivanovic-Burmazovic, Ivana
通讯作者：
Ivanovic-Burmazovic, Ivana
影响因子：
3.9
作者：
Koppenol, Willem H.;Bounds, Patricia L.
通讯作者：
Bounds, Patricia L.
影响因子：
4.6
作者：
Ivanovic-Burmazovic, Ivana;Filipovic, Milos R.
通讯作者：
Filipovic, Milos R.
影响因子：
7.3
作者：
Ali, M. Y.;Ping, C. Y.;Moore, P. K.
通讯作者：
Moore, P. K.
影响因子：
1.8
作者：
HART, TW;VINE, MB;WALDEN, NR
通讯作者：
WALDEN, NR