Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection.
Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection.
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玉米 AKINβγ 蛋白与水稻黑条矮缩病毒 P8 相互作用并抑制病毒感染
DOI：
10.3390/v12121387
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发表时间：
2020-12-04
期刊：
影响因子：
--
通讯作者：
Fan Z
中科院分区：
文献类型：
--
作者：
Li M;Sun X;Di D;Zhang A;Qing L;Zhou T;Miao H;Fan Z
水稻黑条矮缩病毒（RBSDV）是引起玉米粗缩病的重要病原体，但对RBSDV侵染的寄主因子了解甚少。为了揭示RBSDV与玉米之间的分子相互作用，以RBSDV P8蛋白为诱饵，对玉米cDNA文库进行酵母双杂交筛选。ZmAKINβγ-1和ZmAKINβγ-2（玉米中拟南芥SNF 1激酶同源物的βγ亚基）具有高度序列相似性（由两个基因拷贝编码），被鉴定为相互作用伴侣。双分子荧光互补分析证实了它们与P8在本氏烟草细胞和玉米原生质体中的相互作用。ZmAKINβγ mRNA的积累水平在病毒症状开始出现时上调，然后下调。ZmAKINβγs是SnRK 1复合物的假定调节亚基，SnRK 1复合物是能量稳态的核心调节剂。玉米中ZmAKINβγs的敲低调节参与糖合成或降解的基因的表达水平，以及葡萄糖和蔗糖的含量。重要的是，ZmAKINβγs表达的下调促进了RBSDV在玉米中的积累。这些结果暗示ZmAKINβγs在调节初级碳水化合物代谢和防御RBSDV感染中的作用。
Rice black streaked dwarf virus (RBSDV) is an important agent causing maize rough dwarf disease, whereas the host factors responding to RBSDV infection are poorly understood. To uncover the molecular interactions between RBSDV and maize, a yeast two-hybrid screen of a maize cDNA library was carried out using the viral P8 protein as a bait. ZmAKINβγ-1 and ZmAKINβγ-2 (βγ subunit of Arabidopsis SNF1 kinase homolog in maize) possessing high sequence similarities (encoded by two gene copies) were identified as interaction partners. Their interactions with P8 were confirmed in both Nicotiana benthamiana cells and maize protoplasts by bimolecular fluorescence complementation assay. The accumulation levels of ZmAKINβγ mRNAs were upregulated at the stage of the viral symptoms beginning to appear and then downregulated. ZmAKINβγs are putative regulatory subunits of the SnRK1 complex, a core regulator for energy homeostasis. Knockdown of ZmAKINβγs in maize regulated the expression levels of the genes involved in sugar synthesis or degradation, and also the contents of both glucose and sucrose. Importantly, downregulation of ZmAKINβγs expressions facilitated the accumulation of RBSDV in maize. These results implicate a role of ZmAKINβγs in the regulation of primary carbohydrate metabolism, and in the defense against RBSDV infection.
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影响因子：
5.6
作者：
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通讯作者：
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3.5
作者：
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影响因子：
11.6
作者：
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通讯作者：
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