Long-read genome assemblies for the study of chromosome expansion: Drosophila kikkawai, Drosophila takahashii, Drosophila bipectinata, and Drosophila ananassae.

Long-read genome assemblies for the study of chromosome expansion: Drosophila kikkawai, Drosophila takahashii, Drosophila bipectinata, and Drosophila ananassae.
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DOI:
10.1093/g3journal/jkad191
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发表时间:
2023-09-30
期刊:
G3 (Bethesda, Md.)
影响因子:
--
通讯作者:
Ellison CE
Ellison CE
中科院分区:
其他
文献类型:
--
作者:
Leung W;Torosin N;Cao W;Reed LK;Arrigo C;Elgin SCR;Ellison CE

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流式细胞术对不同种类果蝇基因组大小的估计显示出3倍的差异,从巯基果蝇的~ 127 Mb到胞瘤果蝇的~ 400 Mb不等。然而,Muller F元件的组装部分(与果蝇的第4条染色体同源)在大小上显示出近14倍的差异,范围从1.3 Mb到bb0 18 Mb。在这里,我们展示了4种果蝇物种的染色体水平长读基因组组装,其扩展的F元件的大小范围从2.3 Mb到20.5 Mb。每个Muller元件在每个组装中作为单个支架存在。这些组合将使人们对染色体大小扩展的进化原因和后果有新的认识。
Flow cytometry estimates of genome sizes among species of Drosophila show a 3-fold variation, ranging from ∼127 Mb in Drosophila mercatorum to ∼400 Mb in Drosophila cyrtoloma. However, the assembled portion of the Muller F element (orthologous to the fourth chromosome in Drosophila melanogaster) shows a nearly 14-fold variation in size, ranging from ∼1.3 Mb to >18 Mb. Here, we present chromosome-level long-read genome assemblies for 4 Drosophila species with expanded F elements ranging in size from 2.3 to 20.5 Mb. Each Muller element is present as a single scaffold in each assembly. These assemblies will enable new insights into the evolutionary causes and consequences of chromosome size expansion.
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