MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells.
MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells.
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MicroRNA-302b 通过靶向人肝细胞癌 SMMC-7721 细胞中的 EGFR 抑制细胞增殖
DOI：
10.1186/1471-2407-13-448
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发表时间：
2013-10-02
期刊：
影响因子：
3.8
通讯作者：
Huang C
中科院分区：
文献类型：
--
作者：
Wang L;Yao J;Shi X;Hu L;Li Z;Song T;Huang C
背景MicroRNA是一种在肿瘤发生中起重要作用的调节因子。虽然miR-302家族被认为是人类癌症中的肿瘤抑制因子，但它们抑制肿瘤发展的机制仍有待确定。方法采用定量逆转录-聚合酶链反应（QRT-PCR）检测27例肝癌组织及其相应癌旁肝组织中miR-302 b和EGFR的表达。采用MTT法、集落形成法、免疫荧光染色法和细胞周期分析法检测miR 302 b对细胞增殖的抑制作用。进行荧光素酶测定以评估EGFR是miR-302 b的新靶标。Western blot检测miR-302 b在27对临床HCC和非肿瘤组织中的表达水平，结果发现miR-302 b表达下调，而EGFR表达上调。双荧光素酶报告基因检测显示EGFR是miR-302 b的新靶点。miR-302 b的重新表达导致肝癌SMMC-7721细胞的增殖抑制。miR-302 b或siEGFR对EGFR的沉默可导致增殖相关蛋白如AKT 2、CCND 1和CDK 2的表达下调。结论miR-302 b可能通过靶向EGFR/AKT 2/CCND 1通路抑制HCC的生长。
BackgroundMicroRNAs are regulators that can play an essential role in tumorigenesis. Although miR-302 families have been suggested to be tumor repressors in human cancer, the mechanism by which they suppress tumor development remains to be defined. In this study, we discover that miR302b suppresses tumor proliferation may due to directly targeting EGFR in human hepatocellular carcinoma (HCC).MethodsQRT-PCR was used to assess miR-302b and EGFR expression in 27 pairs of clinical hepatocellular carcinoma tissues and their corresponding adjacent nontumorous liver tissues. MTT, colony formation, immunofluorescence staining, and cell cycle assays were used to examine the tumor suppressor role of miR302b in cell proliferation. Luciferase assays were performed to assess the EGFR was a novel target of miR-302b. Western blot assay was used to validate the protein expression level.ResultsWe demonstrated that miR-302b was frequently down-regulated, whereas EGFR was up-regulated in 27 pairs of clinical HCC and non-tumorous counterparts. The dual-luciferase reporter assays revealed that EGFR was a novel target of miR-302b. Re-expression of miR-302b resulted in the inhibition of proliferation in hepatocellular carcinoma SMMC-7721 cells. The silencing of EGFR by miR-302b or siEGFR led to down-regulation of proliferation-related proteins, such as AKT2, CCND1, and CDK2.ConclusionmiR-302b suppresses HCC growth may due to targeting the EGFR/AKT2/CCND1 pathway.
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影响因子：
4.5
作者：
Lin, Shi-Lung;Chang, Donald C.;Ying, Shao-Yao
通讯作者：
Ying, Shao-Yao
DOI：
10.1016/j.bbrc.2003.08.032
发表时间：
2003-09-26
影响因子：
3.1
作者：
Shi, CJ;Yu, LZ;Zeng, HH
通讯作者：
Zeng, HH
天然药物对 microRNA 的调节：化学预防和化疗研究的新兴领域。
影响因子：
3.7
作者：
Li, Yiwei;Kong, Dejuan;Wang, Zhiwei;Sarkar, Fazlul H.
通讯作者：
Sarkar, Fazlul H.
影响因子：
11.2
作者：
Sachdeva, Mohit;Mo, Yin-Yuan
通讯作者：
Mo, Yin-Yuan
影响因子：
7.5
作者：
Negrini, Massimo;Nicoloso, Milena S.;Calin, George A.
通讯作者：
Calin, George A.