Transcriptomic profiling of tall fescue in response to heat stress and improved thermotolerance by melatonin and 24-epibrassinolide.
Transcriptomic profiling of tall fescue in response to heat stress and improved thermotolerance by melatonin and 24-epibrassinolide.
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DOI：
10.1186/s12864-018-4588-y
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发表时间：
2018-03-27
期刊：
影响因子：
4.4
通讯作者：
Chan Z
中科院分区：
文献类型：
--
作者：
Alam MN;Zhang L;Yang L;Islam MR;Liu Y;Luo H;Yang P;Wang Q;Chan Z
高羊茅是一种广泛使用的冷季型草坪草，对高温相对敏感。据报道，褪黑素（MT）和24-表油菜素内酯（EBL）等化合物可以有效提高植物的热胁迫耐受性。在这项研究中，我们报道了MT和EBL预处理的高羊茅幼苗在热胁迫条件下表现出活性氧（ROS）、电解质渗漏（EL）和丙二醛（MDA）减少，但叶绿素（Chl）、总蛋白和抗氧化酶活性增加，从而改善植物生长。转录组分析显示，热处理 2 小时和 12 小时后，分别有 4311 个和 8395 个 unigene 发生显着变化。其中，涉及热应激反应、DNA、RNA和蛋白质降解、氧化还原、能量代谢和激素代谢途径的基因在热应激后被高度诱导。 MT 和 EBL 处理均显着上调 FaHSFA3、FaAWPM 和 FaCYTC2 等基因，表明这些基因可能作为 MT 和 EBL 的假定靶基因。这些发现表明，热应激引起高羊茅的广泛转录组重编程，并且外源应用MT和EBL有效地提高了高羊茅的耐热性。本文的在线版本 (10.1186/s12864-018-4588-y) 包含补充材料，可供授权用户使用。
Tall fescue is a widely used cool season turfgrass and relatively sensitive to high temperature. Chemical compounds like melatonin (MT) and 24-epibrassinolide (EBL) have been reported to improve plant heat stress tolerance effectively. In this study, we reported that MT and EBL pretreated tall fescue seedlings showed decreased reactive oxygen species (ROS), electrolyte leakage (EL) and malondialdehide (MDA), but increased chlorophyll (Chl), total protein and antioxidant enzyme activities under heat stress condition, resulting in improved plant growth. Transcriptomic profiling analysis showed that 4311 and 8395 unigenes were significantly changed after 2 h and 12 h of heat treatments, respectively. Among them, genes involved in heat stress responses, DNA, RNA and protein degradation, redox, energy metabolisms, and hormone metabolism pathways were highly induced after heat stress. Genes including FaHSFA3, FaAWPM and FaCYTC2 were significantly upregulated by both MT and EBL treatments, indicating that these genes might function as the putative target genes of MT and EBL. These findings indicated that heat stress caused extensively transcriptomic reprogramming of tall fescue and exogenous application of MT and EBL effectively improved thermotolerance in tall fescue. The online version of this article (10.1186/s12864-018-4588-y) contains supplementary material, which is available to authorized users.
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影响因子：
10.3
作者：
DUBBELS, R;REITER, RJ;SCHLOOT, W
通讯作者：
SCHLOOT, W
DOI：
10.1071/pp9850201
发表时间：
1985-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
HEWITT, FR;HOUGH, T;ROWAN, KS
通讯作者：
ROWAN, KS
影响因子：
13.8
作者：
Chan, Zhulong;Bigelow, Patrick J.;Grumet, Rebecca
通讯作者：
Grumet, Rebecca
影响因子：
4.6
作者：
Caño-Delgado, A;Yin, YH;Chory, J
通讯作者：
Chory, J
影响因子：
5.6
作者：
Hu T;Liu SQ;Amombo E;Fu JM
通讯作者：
Fu JM