An Unbiased cDNA Library Prepared from Isolated Aplysia Sensory Neuron Processes Is Enriched for Cytoskeletal and Translational mRNAs
An Unbiased cDNA Library Prepared from Isolated Aplysia Sensory Neuron Processes Is Enriched for Cytoskeletal and Translational mRNAs
复制标题
从分离的海兔感觉神经元过程中制备的无偏 cDNA 文库富含细胞骨架和翻译 mRNA
作者：
R. Moccia;Dillon Y. Chen;Vlasta Lyles;Estreya Kapuya;Yaping E;S. Kalachikov;C. Spahn;J. Frank;E. Kandel;M. Barad;K. Martin
在培养的海兔感觉运动型突触中，局部蛋白质合成是保持突触特异性可塑性所必需的。为了鉴定突触定位的mRNAs，我们从分离的感觉神经突起中制备了一个c DNA文库。通过序列分析，我们估计该文库包含263个不同的mRNAs，其中98个mRNAs占所有克隆的70%。本地化的转录本富含编码细胞骨架元件和翻译机制组件的mRNAs。原位杂交证实，至少有8个转录本的mRNAs存在于远端神经突起中。免疫细胞化学显示，5-羟色胺调节编码α1-微管蛋白的一种局部mRNAs的翻译。我们对编码细胞骨架元件的mRNAs的鉴定表明，局部蛋白质合成是与持续突触加强相关的新突触连接生长所必需的。我们对编码翻译机制组成部分的mRNAs的发现表明，局部蛋白质合成有助于提高突触的翻译能力。
Local protein synthesis is required for long-lasting synapse-specific plasticity in cultured Aplysia sensorimotor synapses. To identify synaptically localized mRNAs, we prepared a cDNA library from isolated sensory neurites. By sequence analysis, we estimate that the library contains 263 distinct mRNAs, with 98 of these mRNAs constituting 70% of all clones. The localized transcripts are enriched for mRNAs encoding cytoskeletal elements and components of the translational machinery. In situ hybridization confirms that the mRNAs for at least eight of these transcripts are present in distal neurites. Immunocytochemistry reveals that serotonin regulates the translation of one of the localized mRNAs, that encoding α1-tubulin. Our identification of mRNAs encoding cytoskeletal elements suggests that local protein synthesis is required for the growth of new synaptic connections associated with persistent synaptic strengthening. Our finding of mRNAs encoding components of the translational machinery suggests that local protein synthesis serves to increase the translational capacity of synapses.
影响因子：
11.4
作者：
NIELSEN, PJ;TRACHSEL, H
通讯作者：
TRACHSEL, H
影响因子：
56.9
作者：
Ban, N;Nissen, P;Steitz, TA
通讯作者：
Steitz, TA
影响因子：
56.9
作者：
BAILEY, CH;CHEN, M
通讯作者：
CHEN, M