Analysis of SARS-CoV-2 viral loads in stool samples and nasopharyngeal swabs from COVID-19 patients in the United Arab Emirates.
Analysis of SARS-CoV-2 viral loads in stool samples and nasopharyngeal swabs from COVID-19 patients in the United Arab Emirates.
复制标题
DOI：
10.1371/journal.pone.0274961
复制
发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
2019年冠状病毒病(新冠肺炎)最先在呼吸道样本中发现，并被发现通常会导致咳嗽和肺炎。然而，包括胃肠道疾病在内的非呼吸道症状也存在，很大比例的患者在很长一段时间内粪便病毒检测呈阳性。在这项横断面研究中，我们调查了粪便和鼻咽拭子中的病毒载量趋势，以及它们与多种人口统计学和临床因素的相关性。这项研究包括211例实验室确诊的轻度埃博拉患者，他们在阿联酋的一个非医院中心完成了隔离期。通过标准化问卷和从患者的病历中收集人口统计学和临床信息。在211名参与者中，25%在本研究进行时两种样本类型的检测均为阴性，其余53%的患者粪便中可检测到病毒RNA。粪便病毒检测阳性与男性性别、腹泻症状和感染期间住院有关。症状出现延迟与粪便检测阳性之间也存在正相关。粪便病毒载量与超重、运动、最近3个月服用抗生素、O型血呈正相关，鼻咽拭子病毒载量以A型血、恒河猴阳性(Rh因子)较高。回归分析表明，在任何给定的患者中，粪便和鼻咽样本中测得的病毒载量之间没有相关性。这项工作的结果突出了与每个样本中更高的病毒计数相关的因素。同时也说明了粪便标本分析对新冠肺炎康复者的随访和诊断的重要性。
Coronavirus disease 2019 (COVID-19) was first identified in respiratory samples and was found to commonly cause cough and pneumonia. However, non-respiratory symptoms including gastrointestinal disorders are also present and a big proportion of patients test positive for the virus in stools for a prolonged period. In this cross-sectional study, we investigated viral load trends in stools and nasopharyngeal swabs and their correlation with multiple demographic and clinical factors. The study included 211 laboratory-confirmed cases suffering from a mild form of the disease and completing their isolation period at a non-hospital center in the United Arab Emirates. Demographic and clinical information was collected by standardized questionnaire and from the medical records of the patient. Of the 211 participants, 25% tested negative in both sample types at the time of this study and 53% of the remaining patients had detectable viral RNA in their stools. A positive fecal viral test was associated with male gender, diarrhea as a symptom, and hospitalization during infection. A positive correlation was also observed between a delayed onset of symptoms and a positive stool test. Viral load in stools positively correlated with, being overweight, exercising, taking antibiotics in the last 3 months and blood type O. The viral load in nasopharyngeal swabs, on the other hand, was higher for blood type A, and rhesus positive (Rh factor). Regression analysis showed no correlation between the viral loads measured in stool and nasopharyngeal samples in any given patient. The results of this work highlight the factors associated with a higher viral count in each sample. It also shows the importance of stool sample analysis for the follow-up and diagnosis of recovering COVID-19 patients.
登录
查看更多内容
SARS-COV-2血统的估计可传播和影响B.1.1.7在英国。
DOI：
10.1126/science.abg3055
发表时间：
2021-04-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Davies NG;Abbott S;Barnard RC;Jarvis CI;Kucharski AJ;Munday JD;Pearson CAB;Russell TW;Tully DC;Washburne AD;Wenseleers T;Gimma A;Waites W;Wong KLM;van Zandvoort K;Silverman JD;CMMID COVID-19 Working Group;COVID-19 Genomics UK (COG-UK) Consortium;Diaz-Ordaz K;Keogh R;Eggo RM;Funk S;Jit M;Atkins KE;Edmunds WJ
通讯作者：
Edmunds WJ
影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
肠道微生物群与 COVID-19 的相互作用揭示了中东人群宿主脂质代谢的联系。
影响因子：
5.2
作者：
Al Bataineh MT;Henschel A;Mousa M;Daou M;Waasia F;Kannout H;Khalili M;Kayasseh MA;Alkhajeh A;Uddin M;Alkaabi N;Tay GK;Feng SF;Yousef AF;Alsafar HS
通讯作者：
Alsafar HS
影响因子：
7.3
作者：
Ding, YQ;Wang, HJ;Yao, KT
通讯作者：
Yao, KT
影响因子：
4.6
作者：
Tayoun AA;Loney T;Khansaheb H;Ramaswamy S;Harilal D;Deesi ZO;Varghese RM;Al Suwaidi H;Alkhajeh A;AlDabal LM;Uddin M;Hamoudi R;Halwani R;Senok A;Hamid Q;Nowotny N;Alsheikh-Ali A
通讯作者：
Alsheikh-Ali A