Irisin-immunoreactivity in neural and non-neural cells of the rodent.
Irisin-immunoreactivity in neural and non-neural cells of the rodent.
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DOI：
10.1016/j.neuroscience.2013.02.050
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发表时间：
2013-06-14
期刊：
影响因子：
3.3
通讯作者：
Dun, N. J.
中科院分区：
文献类型：
--
作者：
Dun, S. L.;Lyu, R. -M.;Chen, Y. -H.;Chang, J. -K.;Luo, J. J.;Dun, N. J.
关键词：
鸢尾素是最近发现的一种肌肉因子，是运动时肌肉分泌的。在大鼠和小鼠中，使用针对鸢尾素肽片段 (42-112) 的抗血清进行的免疫组织化学研究表明，在三种类型的细胞中检测到了鸢尾素免疫反应性 (irIRN)；即骨骼肌细胞、心肌细胞和小脑的浦肯野细胞。用预吸收鸢尾素肽 (42-112) (1 μg/ml) 的鸢尾素抗血清处理的组织切片没有显示出免疫反应性。小脑浦肯野细胞也用针对包含 5 (FNDC5)（鸢尾素前体蛋白）的纤连蛋白 II 型结构域的抗血清进行了免疫标记。用鸢尾素抗血清和谷氨酸脱羧酶（GAD）抗体对小脑切片进行双重标记，结果显示几乎所有 irIRN 浦肯野细胞均为 GAD 阳性。将荧光示踪剂 Fluorogold 注射到大鼠髓质的前庭核中，逆行标记了一群浦肯野细胞，其中一些也是 irIRN。我们的结果提供了 irIRN 在啮齿动物骨骼和心肌以及大脑中表达的第一个证据，其中 irIRN 存在于小脑的 GAD 阳性浦肯野细胞中。我们的发现与其他人的报告一起使我们假设一种新的神经通路，它起源于小脑浦肯野细胞，通过髓质和脊髓中的几个中间突触，调节脂肪细胞代谢。
Irisin is a recently identified myokine secreted from the muscle in response to exercise. In the rats and mice, immunohistochemical studies with an antiserum against irisin peptide fragment (42–112), revealed that irisin-immunoreactivity (irIRN) was detected in three types of cells; namely, skeletal muscle cells, cardiomyocytes, and Purkinje cells of the cerebellum. Tissue sections processed with irisin antiserum pre-absorbed with the irisin peptide(42–112) (1 μg/ml) showed no immunoreactivity. Cerebellar Purkinje cells were also immunolabeled with an antiserum against fibronectin type II domain containing 5 (FNDC5), the precursor protein of irisin. Double-labeling of cerebellar sections with irisin antiserum and glutamate decarboxylase (GAD) antibody showed that nearly all irIRN Purkinje cells were GAD-positive. Injection of the fluorescence tracer Fluorogold into the vestibular nucleus of the rat medulla retrogradely labeled a population of Purkinje cells, some of which were also irIRN. Our results provide the first evidence of expression of irIRN in the rodent skeletal and cardiac muscle, and in the brain where it is present in GAD-positive Purkinje cells of the cerebellum. Our findings together with reports by others led us to hypothesize a novel neural pathway, which originates from cerebellum Purkinje cells, via several intermediary synapses in the medulla and spinal cord, and regulates adipocyte metabolism.
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影响因子：
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作者：
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影响因子：
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