The effect of ultrasound-related stimuli on cell viability in microfluidic channels.
The effect of ultrasound-related stimuli on cell viability in microfluidic channels.
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DOI：
10.1186/1477-3155-11-20
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发表时间：
2013-06-28
影响因子：
10.2
通讯作者：
Hill M
中科院分区：
文献类型：
--
作者：
Ankrett DN;Carugo D;Lei J;Glynne-Jones P;Townsend PA;Zhang X;Hill M
在超声微装置中，已知造影剂微泡会引发细胞局部的空化和流动，从而可能损害细胞活力。在这里，我们通过省略造影剂并监测中度至极端超声相关刺激下的细胞活力来研究单独超声的效果。将悬浮的 H9c2 心脏成肌细胞暴露于玻璃微毛细管内的超声波场，并在不同的超声相关刺激下监测其活力。确定最佳注射流速为 2.6 mL/h，可保持高活力 (~95%)，并且对细胞没有明显的机械应力。该流速还允许细胞充分暴露于超声，以诱导生物效应（约 5 秒），同时提供经济的样品收集和处理时间。尽管在最大实验电压 (29 Vpp) 下传感器温度从环境温度 23°C 增加到 54°C，但计算流体动力学模拟和控制（没有 US）表明，压力节点平面中的单元介质温度不超过 34°C。在固定频率扫描周期（tsw = 0.05 秒）下，将细胞暴露于 0-29 Vpp 的 US 振幅下，结果表明，在高达 ~15 Vpp 的情况下，活力受到的影响最小。在 21 Vpp 时，活力下降约 17%，对应于类瑞利流的开始；在 29 Vpp 时，活力下降约 60%，与流速度增加或空化的潜在开始相对应。在固定振幅 (29 Vpp) 和不同频率扫描周期 (tsw = 0.02-0.50 秒) 下，细胞活力在 tsw ≥ 0.08 秒时保持相对恒定，而在 tsw < 0.08 秒时活力降低，在 tsw = 0.05 秒时记录最低活力。 CA 的缺失使我们能够研究单独使用 US 对细胞活力的影响。与美国相关的中度至极度细胞刺激使我们能够区分维持高活力的刺激和显着降低细胞活力的刺激。这项研究的结果可能会引起美国诱导细胞内药物输送和生物细胞超声操作领域的研究人员的潜在兴趣。
In ultrasonic micro-devices, contrast agent micro-bubbles are known to initiate cavitation and streaming local to cells, potentially compromising cell viability. Here we investigate the effects of US alone by omitting contrast agent and monitoring cell viability under moderate-to-extreme ultrasound-related stimuli. Suspended H9c2 cardiac myoblasts were exposed to ultrasonic fields within a glass micro-capillary and their viability monitored under different US-related stimuli. An optimal injection flow rate of 2.6 mL/h was identified in which, high viability was maintained (~95%) and no mechanical stress towards cells was evident. This flow rate also allowed sufficient exposure of cells to US in order to induce bioeffects (~5 sec), whilst providing economical sample collection and processing times. Although the transducer temperature increased from ambient 23°C to 54°C at the maximum experimental voltage (29 Vpp), computational fluid dynamic simulations and controls (absence of US) revealed that the cell medium temperature did not exceed 34°C in the pressure nodal plane. Cells exposed to US amplitudes ranging from 0–29 Vpp, at a fixed frequency sweep period (tsw = 0.05 sec), revealed that viability was minimally affected up to ~15 Vpp. There was a ~17% reduction in viability at 21 Vpp, corresponding to the onset of Rayleigh-like streaming and a ~60% reduction at 29 Vpp, corresponding to increased streaming velocity or the potential onset of cavitation. At a fixed amplitude (29 Vpp) but with varying frequency sweep period (tsw = 0.02-0.50 sec), cell viability remained relatively constant at tsw ≥ 0.08 sec, whilst viability reduced at tsw < 0.08 sec and minimum viability recorded at tsw = 0.05 sec. The absence of CA has enabled us to investigate the effect of US alone on cell viability. Moderate-to-extreme US-related stimuli of cells have allowed us to discriminate between stimuli that maintain high viability and stimuli that significantly reduce cell viability. Results from this study may be of potential interest to researchers in the field of US-induced intracellular drug delivery and ultrasonic manipulation of biological cells.
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影响因子：
6.1
作者：
Le Gac, Severine;Zwaan, Ed;Ohl, Claus-Dieter
通讯作者：
Ohl, Claus-Dieter
影响因子：
3.2
作者：
Carugo, Dario;Ankrett, Dyan N.;Hill, Martyn
通讯作者：
Hill, Martyn
影响因子：
2.9
作者：
Hultstrom, J.;Manneberg, O.;Wiklund, M.
通讯作者：
Wiklund, M.
影响因子：
4.2
作者：
Collis, James;Manasseh, Richard;Zhu, Yonggang
通讯作者：
Zhu, Yonggang
影响因子：
6.1
作者：
Longsine-Parker, Whitney;Wang, Han;Han, Arum
通讯作者：
Han, Arum