DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings.
DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings.
复制标题
DNA 损伤水平决定苜蓿幼苗中的环丁基嘧啶二聚体修复机制。
DOI：
10.1105/tpc.6.11.1635
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
B. M. Sutherland
中科院分区：
文献类型：
--
作者：
F. E. Quaite;Shinnosuke Takayanagi;J. Ruffini;John C. Sutherland;B. M. Sutherland
阳光中的紫外线辐射会损伤植物的DNA，但对其类型、损伤能力和修复途径的协调了解甚少。我们用不同剂量的紫外线照射完整的紫花苜蓿幼苗，诱导不同初始水平的环丁基嘧啶二聚体，并通过切除和光再激活测量修复。对经环丁基嘧啶二聚体特异性紫外核酸内切酶处理的非放射性DNA，采用碱性凝胶电泳技术，通过电子成像定量测定乙二染色DNA的数量，并根据平均分子长度计算病变频率。在较低的初始二聚体频率下（每百万碱基少于30个二聚体），幼苗仅使用光活化来修复二聚体；切除修复无显著性差异。在较高的损伤水平，切除和光修复都有显著贡献。这种策略将允许低损伤水平的植物使用只需要外部光能的无差错修复，而遭受更高损伤频率的幼苗可以调用额外的修复过程，需要细胞能量。因此，表征植物的修复需要对一系列条件进行调查，包括初始损伤的水平。
Ultraviolet radiation in sunlight damages DNA in plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels of cyclobutyl pyrimidine dimers and measured repair by excision and photoreactivation. By using alkaline gel electrophoresis of nonradioactive DNAs treated with a cyclobutyl pyrimidine dimer-specific UV endonuclease, we quantitated ethidium-stained DNA by electronic imaging and calculated lesion frequencies from the number average molecular lengths. At low initial dimer frequencies (less than ~30 dimers per million bases), the seedlings used only photoreactivation to repair dimers; excision repair was not significant. At higher damage levels, both excision and photorepair contributed significantly. This strategy would allow plants with low damage levels to use error-free repair requiring only an external light energy source, whereas seedlings subjected to higher damage frequencies could call on additional repair processes requiring cellular energy. Characterization of repair in plants thus requires an investigation of a range of conditions, including the level of initial damage.
非放射性 DNA 中超螺旋 DNA 裂解的定量：在电离辐射和合成核酸内切酶裂解中的应用。
影响因子：
2.9
作者：
Sutherland,BM;Bennett,PV;Conlon,K;Epling,GA;Sutherland,JC
通讯作者：
Sutherland,JC
影响因子：
2.9
作者：
John C. Sutherland;Bohai Lin;D. Monteleone;JoAnn H. Mugavero;B. M. Sutherland;J. Trunk
通讯作者：
John C. Sutherland;Bohai Lin;D. Monteleone;JoAnn H. Mugavero;B. M. Sutherland;J. Trunk
琼脂糖凝胶中单链和双链 DNA 的单向脉冲场电泳：与迁移率和分子长度相关的分析表达式及其在链断裂测量中的应用。
影响因子：
2.9
作者：
Sutherland,JC;Monteleone,DC;Mugavero,JH;Trunk,J
通讯作者：
Trunk,J