The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors.
The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors.
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DOI：
10.1083/jcb.200806009
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发表时间：
2008-11-03
期刊：
影响因子：
--
通讯作者：
Arshavsky VY
中科院分区：
文献类型：
--
作者：
Baker SA;Haeri M;Yoo P;Gospe SM 3rd;Skiba NP;Knox BE;Arshavsky VY
光感受器是区室化的神经元，其中所有负责唤起视觉信号的蛋白质都局限于外节。然而，负责建立和维持感光细胞区室化的机制知之甚少。在这里，我们研究了两个相关的膜蛋白，R9AP和syntaxin 3，一个居住在和其他排除在外段的目标。令人惊讶的是，我们发现只有syntaxin 3在其序列中编码有靶向信息，并且其去除将该蛋白质重定向到外部片段。此外，内质网和线粒体中的蛋白质在去除它们的靶向信号后同样被重定向到外节。这揭示了一种模式，即缺乏特异性靶向信息的膜蛋白被传递到外节，这可能反映了这种细胞器对不断更新所必需的新材料的巨大胃口。这也意味着每一个位于外节之外的蛋白质都必须有一种方法来避免这种“默认”的运输流。
Photoreceptors are compartmentalized neurons in which all proteins responsible for evoking visual signals are confined to the outer segment. Yet, the mechanisms responsible for establishing and maintaining photoreceptor compartmentalization are poorly understood. Here we investigated the targeting of two related membrane proteins, R9AP and syntaxin 3, one residing within and the other excluded from the outer segment. Surprisingly, we have found that only syntaxin 3 has targeting information encoded in its sequence and its removal redirects this protein to the outer segment. Furthermore, proteins residing in the endoplasmic reticulum and mitochondria were similarly redirected to the outer segment after removing their targeting signals. This reveals a pattern where membrane proteins lacking specific targeting information are delivered to the outer segment, which is likely to reflect the enormous appetite of this organelle for new material necessitated by its constant renewal. This also implies that every protein residing outside the outer segment must have a means to avoid this “default” trafficking flow.
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影响因子：
1.8
作者：
Concepcion, F;Mendez, A;Chen, J
通讯作者：
Chen, J
DOI：
10.1073/pnas.95.18.10620
发表时间：
1998-09-01
影响因子：
11.1
作者：
Deretic, D;Schmerl, S;McDowell, JH
通讯作者：
McDowell, JH
DOI：
10.1073/pnas.0500095102
发表时间：
2005-03-01
影响因子：
11.1
作者：
Deretic, D;Williams, AH;Arendt, A
通讯作者：
Arendt, A
影响因子：
3.5
作者：
Keresztes, G;Mutai, H;Heller, S
通讯作者：
Heller, S
影响因子：
64.5
作者：
Chuang, Jen-Zen;Zhao, Yu;Sung, Ching-Hwa
通讯作者：
Sung, Ching-Hwa