Honeybee (Apis mellifera)-associated bacterial community affected by American foulbrood: detection of Paenibacillus larvae via microbiome analysis.
Honeybee (Apis mellifera)-associated bacterial community affected by American foulbrood: detection of Paenibacillus larvae via microbiome analysis.
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DOI：
10.1038/s41598-017-05076-8
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发表时间：
2017-07-11
影响因子：
4.6
通讯作者：
Hubert J
中科院分区：
文献类型：
--
作者：
Erban T;Ledvinka O;Kamler M;Nesvorna M;Hortova B;Tyl J;Titera D;Markovic M;Hubert J
蜜蜂（Apis mellifera L.）工蜂是引起检疫性疾病美国幼虫病（AFB）的幼虫类芽孢杆菌孢子的被动媒介。我们使用16 S rRNA基因的元条形码分析评估了蜜蜂微生物组中幼虫的相对比例。在AFB区外的工作人员（对照-AFB 0）、来自AFB养蜂场无症状菌落的工作人员（AFB 1）和来自表现出临床AFB症状的菌落的工作人员（AFB 2）中分析了微生物组。在去除核心细菌序列后，处理整个群落的微生物组和包含病原性/环境细菌的截止微生物组;在统计分析中考虑静脉曲张水平。AFB状态和静脉曲张水平之间没有观察到相关性，但AFB影响工蜂细菌群落，主要是病原菌/环境细菌。在AFB 1和AFB 0菌落之间，P.幼虫的相对丰度没有显著差异，但我们确实观察到AFB 2中的P.幼虫丰度相对于AFB 1中的丰度增加了9倍。AFB 2和AFB 1中弗氏柠檬酸杆菌和蜂房哈夫尼菌的相对序列数高于AFB 0，而粪肠球菌、产酸克雷伯菌、意大利螺原体和摩氏摩根菌的相对序列数高于AFB 0和AFB 1。
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA gene. The microbiome was analyzed in workers outside of the AFB zone (control - AFB0), in workers from asymptomatic colonies in an AFB apiary (AFB1), and in workers from colonies exhibiting clinical AFB symptoms (AFB2). The microbiome was processed for the entire community and for a cut-off microbiome comprising pathogenic/environmental bacteria following the removal of core bacterial sequences; varroosis levels were considered in the statistical analysis. No correlation was observed between AFB status and varroosis level, but AFB influenced the worker bee bacterial community, primarily the pathogenic/environmental bacteria. There was no significant difference in the relative abundance of P. larvae between the AFB1 and AFB0 colonies, but we did observe a 9-fold increase in P. larvae abundance in AFB2 relative to the abundance in AFB1. The relative sequence numbers of Citrobacter freundii and Hafnia alvei were higher in AFB2 and AFB1 than in AFB0, whereas Enterococcus faecalis, Klebsiella oxytoca, Spiroplasma melliferum and Morganella morganii were more abundant in AFB0 and AFB1 than in AFB2.
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影响因子：
2.4
作者：
Dobbelaere, W;de Graaf, DC;Jacobs, FJ
通讯作者：
Jacobs, FJ
影响因子：
5.7
作者：
Garrido-Bailón E;Higes M;Martínez-Salvador A;Antúnez K;Botías C;Meana A;Prieto L;Martín-Hernández R
通讯作者：
Martín-Hernández R
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
Gillard, M.;Charriere, J. D.;Belloy, L.
通讯作者：
Belloy, L.
影响因子：
4.4
作者：
Kozich, James J.;Westcott, Sarah L.;Schloss, Patrick D.
通讯作者：
Schloss, Patrick D.