Granulocyte-colony stimulating factor in combination with stem cell factor confers greater neuroprotection after hypoxic-ischemic brain damage in the neonatal rats than a solitary treatment.
Granulocyte-colony stimulating factor in combination with stem cell factor confers greater neuroprotection after hypoxic-ischemic brain damage in the neonatal rats than a solitary treatment.
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DOI：
10.1007/s12975-012-0225-2
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Tang, Jiping
中科院分区：
文献类型：
--
作者：
Doycheva, Desislava;Shih, Gary;Chen, Hank;Applegate, Richard;Zhang, John H.;Tang, Jiping
关键词：
新生儿缺氧缺血（HI）是一种破坏性的条件，导致神经细胞死亡，并往往最终在神经功能缺损。粒细胞集落刺激因子（G-CSF）已被证明通过抑制各种中风模型中的细胞凋亡和炎症而具有神经保护活性。干细胞因子（SCF）调节骨髓中的造血干细胞，并已报道在实验性缺血性中风模型中具有神经保护特性。在这项研究中，我们的目的是确定G-CSF联合SCF治疗实验性HI后的保护作用。对7日龄的Sprague-Dawley大鼠进行单侧颈动脉结扎，然后缺氧2.5小时。将动物随机分配到五个组：假手术组（n =8）、溶媒组（n=8）、G-CSF治疗组（n=9）、SCF治疗组（n=9）和G-CSF+SCF治疗组（共给药组; n=10）。HI后1小时腹膜内给予G-CSF（50 µ g/kg）、SCF（50 µg/kg）和G-CSF+SCF（50 µg/kg），随后连续4天每日注射（共注射5次）。HI后14天对动物实施安乐死，以进行神经学测试。此外，还对脑、心脏、肝脏、脾脏和肾脏萎缩进行了评估。G-CSF和G-CSF+SCF治疗在HI后14天时均改善了身体生长并减少了脑萎缩。各组外周器官重量无显著差异。最后，G-CSF+SCF联合给药组显示出神经功能的显著改善。我们的数据表明，G-CSF联合SCF不仅可以预防脑萎缩，而且可以显着改善神经功能。
Neonatal hypoxia-ischemia (HI) is a devastating condition resulting in neuronal cell death and often culminates in neurological deficits. Granulocyte-colony stimulating factor (G-CSF) has been shown to have neuroprotective activity via inhibition of apoptosis and inflammation in various stroke models. Stem cell factor (SCF) regulates hematopoietic stem cells in the bone marrow and has been reported to have neuroprotective properties in an experimental ischemic stroke model. In this study we aim to determine the protective effects of G-CSF in combination with SCF treatment after experimental HI. Seven-day old Sprague-Dawley rats were subjected to unilateral carotid artery ligation followed by 2.5 hours of hypoxia. Animals were randomly assigned to five groups: Sham (n=8), Vehicle (n=8), HI with G-CSF treatment (n=9), HI with SCF treatment (n=9) and HI with G-CSF+SCF treatment (coadministration group; n=10). G-CSF (50 µg/kg), SCF (50 µg/kg) and G-CSF+SCF (50 µg/kg) were administered intraperitoneally 1 hour post HI followed by daily injection for 4 consecutive days (five total injections). Animals were euthanized 14 days after HI for neurological testing. Additionally assessment of brain, heart, liver, spleen and kidney atrophy was performed. Both G-CSF and G-CSF+SCF treatments improved body growth and decreased brain atrophy at 14days post HI. No significant differences were found in the peripheral organ weights between groups. Finally, the G-CSF+SCF coadministration group showed significant improvement in neurological function. Our data suggest that administration of G-CSF in combination with SCF not only prevented brain atrophy but also significantly improved neurological function.
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DOI：
10.1007/978-3-7091-0693-8_44
发表时间：
2011
期刊：
Acta neurochirurgica. Supplement
影响因子：
--
作者：
Khatibi, Nikan H;Jadhav, Vikram;Saidi, Mehdi;Chen, Wanqiu;Martin, Robert;Stier, Gary;Tang, Jiping;Zhang, John H
通讯作者：
Zhang, John H
影响因子：
5
作者：
Feng, YZ;Fratkins, JD;LeBlanc, MH
通讯作者：
LeBlanc, MH
影响因子：
8.3
作者：
GARCIA, JH;WAGNER, S;HU, XJ
通讯作者：
HU, XJ
影响因子：
2.9
作者：
Matchett, Gerald A.;Calinisan, Jason B.;Zhang, John H.
通讯作者：
Zhang, John H.
影响因子：
37.8
作者：
Kawada, H;Takizawa, S;Hotta, T
通讯作者：
Hotta, T