A Tandemly Arranged Pattern of Two 5S rDNA Arrays in Amolops mantzorum (Anura, Ranidae)
A Tandemly Arranged Pattern of Two 5S rDNA Arrays in Amolops mantzorum (Anura, Ranidae)
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Amolops mantzorum（无尾蛙，蛙科）两个 5S rDNA 阵列的串联排列模式
DOI：
10.1159/000464128
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发表时间：
2017-03
影响因子：
1.7
通讯作者：
Zeng Xiaomao
中科院分区：
文献类型：
--
作者：
Liu Ting;Song Menghuan;Xia Yun;Zeng Xiaomao
为了扩大对无尾两栖动物5S rDNA组织结构的认识，利用FISH技术分离、鉴定和定位了无尾无尾两栖动物的5S rDNA序列。在不同种群的标本中发现了两种类型的5S rDNA，即I型(209bp)和II型(约870bp)。它们都含有一个118个碱基的编码序列，很容易通过它们的非转录间隔区(NTS)大小和组成来区分。4个探针(5S rDNA编码序列、I型NTS、II型NTS和III型5S rDNA全序列)分别用Tamra或地高辛标记，与各地样品的有丝分裂染色体杂交。结果表明，所有的探针都显示了在5号染色体的每个着丝粒区域和端粒区域出现的相同信号，没有群体内或群体之间的差异。显然，5S rDNA阵列的类型I和类型II都是串联排列的，这与迄今记录的其他青蛙或鱼类形成了对比。更有趣的是，所有的探针都在所有的核型中检测到着丝粒区域，这表明存在一个来自5S rDNA的卫星DNA家族。
In an attempt to extend the knowledge of the 5S rDNA organization in anurans, the 5S rDNA sequences of Amolops mantzorum were isolated, characterized, and mapped by FISH. Two forms of 5S rDNA, type I (209 bp) and type II (about 870 bp), were found in specimens investigated from various populations. Both of them contained a 118-bp coding sequence, readily differentiated by their non-transcribed spacer (NTS) sizes and compositions. Four probes (the 5S rDNA coding sequences, the type I NTS, the type II NTS, and the entire type II 5S rDNA sequences) were respectively labeled with TAMRA or digoxigenin to hybridize with mitotic chromosomes for samples of all localities. It turned out that all probes showed the same signals that appeared in every centromeric region and in the telomeric regions of chromosome 5, without differences within or between populations. Obviously, both type I and type II of the 5S rDNA arrays arranged in tandem, which was contrasting with other frogs or fishes recorded to date. More interestingly, all the probes detected centromeric regions in all karyotypes, suggesting the presence of a satellite DNA family derived from 5S rDNA.
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