Related polymorphic F-box protein genes between haplotypes clustering in the BAC contig sequences around the S-RNase of Japanese pear.
Related polymorphic F-box protein genes between haplotypes clustering in the BAC contig sequences around the S-RNase of Japanese pear.
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DOI：
10.1093/jxb/erq381
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发表时间：
2011-03
影响因子：
6.9
通讯作者：
Takasaki-Yasuda T
中科院分区：
文献类型：
--
作者：
Okada K;Tonaka N;Taguchi T;Ichikawa T;Sawamura Y;Nakanishi T;Takasaki-Yasuda T
蔷薇科果树的自交不亲和性主要由雌蕊编码核糖核酸酶(S-核糖核酸酶)的S基因和花粉S基因控制。李的花粉S是一个位于S核糖核酸酶附近的F-box蛋白基因(SLf/sfb)，但在梨和苹果中尚未被鉴定。在日本梨中，与S核糖核酸酶连锁的各种F-box蛋白基因(PpSFBB-α-γ)被认为是花粉S的候选基因。构建了日本梨S核糖核酸酶基因附近的两个细菌人工染色体重叠群，分别测定了S4核糖核酸酶基因附近的649 kb和S2核糖核酸酶基因周围的378 kb。发现了6个和10个花粉特异的F-box蛋白基因(分别命名为PpSFBB4-U1-u4、4-d1-d2和PpSFBB2-u1-u5、2-d1-d5)，但缺失PpSFBB4-α-γ和PpSFBB2-γ。PpSFBB4基因与PpSFBB2基因的氨基酸同源性为66.2-93.1%，表明相关的F-box蛋白基因在日本梨S核糖核酸酶附近的单倍型之间聚集。系统发育分析将梨属和苹果属的36个F-box蛋白基因分为两大类(I和II)，并产生了PpSFBB基因和PpSFBB/苹果F-box蛋白基因对。第一组由76.3-94.9%的基因对组成，而第二组由同源性较高的基因对组成(>；92%)。这一分类表明，在第二组中，PpSFBB基因的多态性较低，属于非S花粉基因，而S候选花粉属于第一组PpSFBB基因。
Most fruit trees in the Rosaceae exhibit self-incompatibility, which is controlled by the pistil S gene, encoding a ribonuclease (S-RNase), and the pollen S gene at the S-locus. The pollen S in Prunus is an F-box protein gene (SLF/SFB) located near the S-RNase, but it has not been identified in Pyrus and Malus. In the Japanese pear, various F-box protein genes (PpSFBB-α–γ) linked to the S-RNase are proposed as the pollen S candidate. Two bacterial artificial chromosome (BAC) contigs around the S-RNase genes of Japanese pear were constructed, and 649 kb around S4-RNase and 378 kb around S2-RNase were sequenced. Six and 10 pollen-specific F-box protein genes (designated as PpSFBB4-u1–u4, 4-d1–d2 and PpSFBB2-u1–u5, 2-d1–d5, respectively) were found, but PpSFBB4-α–γ and PpSFBB2-γ were absent. The PpSFBB4 genes showed 66.2–93.1% amino acid identity with the PpSFBB2 genes, which indicated clustering of related polymorphic F-box protein genes between haplotypes near the S-RNase of the Japanese pear. Phylogenetic analysis classified 36 F-box protein genes of Pyrus and Malus into two major groups (I and II), and also generated gene pairs of PpSFBB genes and PpSFBB/Malus F-box protein genes. Group I consisted of gene pairs with 76.3–94.9% identity, while group II consisted of gene pairs with higher identities (>92%) than group I. This grouping suggests that less polymorphic PpSFBB genes in group II are non-S pollen genes and that the pollen S candidates are included in the group I PpSFBB genes.
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
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作者：
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Robbins, TP
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作者：
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通讯作者：
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影响因子：
2.4
作者：
Castillo, C;Takasaki, T;Nakanishi, T
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Nakanishi, T