In vitro generation of an osteochondral construct using injectable hydrogel composites encapsulating rabbit marrow mesenchymal stem cells.
In vitro generation of an osteochondral construct using injectable hydrogel composites encapsulating rabbit marrow mesenchymal stem cells.
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DOI：
10.1016/j.biomaterials.2009.01.048
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发表时间：
2009-05
期刊：
影响因子：
14
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Guo, Xuan;Park, Hansoo;Liu, Guangpeng;Liu, Wei;Cao, Yilin;Tabata, Yasuhiko;Kasper, F. Kurtis;Mikos, Antonios G.
关键词：
将富马酸低聚乙二醇酯(OPF)与明胶微粒(MPS)组成可注射、可生物降解的水凝胶复合材料，构建由成骨层和成骨层组成的双层骨软骨结构，并研究这两层包埋的兔骨髓间充质干细胞(MSCs)的体外分化情况。结果表明，软骨层中的MSCs能够向软骨细胞分化，尤其是在含有转化生长因子-β-1的MPS存在的情况下。在成骨层，细胞维持其成骨表型。尽管成骨层中的钙沉积有限，但成骨层中的细胞显著促进了成骨层中MSCs的成软骨分化。将转化生长因子-β-1负载的MPS包裹MSCs，并在双层结构中与成骨细胞共同培养，效果最好。总之，本研究展示了模拟骨软骨组织结构和功能的双层水凝胶复合材料的制备，以及这些复合材料作为细胞和生长因子载体的应用。
Injectable, biodegradable hydrogel composites of crosslinked oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles (MPs) were utilized to fabricate a bilayered osteochondral construct consisting of a chondrogenic layer and an osteogenic layer, and to investigate the differentiation of rabbit marrow mesenchymal stem cells (MSCs) encapsulated in both layers in vitro. The results showed that MSCs in the chondrogenic layer were able to undergo chondrogenic differentiation, especially in the presence of TGF-β1-loaded MPs. In the osteogenic layer, cells maintained their osteoblastic phenotype. Although calcium deposition in the osteogenic layer was limited, cells in the osteogenic layer significantly enhanced chondrogenic differentiation of MSCs in the chondrogenic layer. The greatest effect was observed when MSCs were encapsulated with TGF-β1-loaded MPs and cultured with osteogenic cells in the bilayered constructs. Overall, this study demonstrates the fabrication of bilayered hydrogel composites that mimic the structure and function of osteochondral tissue, along with the application of these composites as cell and growth factor carriers.
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影响因子：
6.2
作者：
Park, Hansoo;Guo, Xuan;Temenoff, Johnna S.;Tabata, Yasuhiko;Caplan, Arnold I.;Kasper, F. Kurtis;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
影响因子：
10.8
作者：
Holland, TA;Tabata, Y;Mikos, AG
通讯作者：
Mikos, AG
DOI：
10.1016/j.bbrc.2004.06.029
发表时间：
2004-07-30
影响因子：
3.1
作者：
Indrawattana, N;Chen, GP;Bunyaratvej, A
通讯作者：
Bunyaratvej, A
影响因子：
2.9
作者：
Moioli, Eduardo K.;Hong, Liu;Mao, Jeremy J.
通讯作者：
Mao, Jeremy J.
影响因子：
--
作者：
Gomes, ME;Bossano, CM;Mikos, AG
通讯作者：
Mikos, AG