454 pyrosequencing based transcriptome analysis of Zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides.
454 pyrosequencing based transcriptome analysis of Zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides.
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DOI：
10.1186/1471-2164-10-574
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发表时间：
2009-12-02
期刊：
影响因子：
4.4
通讯作者：
Bak S
中科院分区：
文献类型：
--
作者：
Zagrobelny M;Scheibye-Alsing K;Jensen NB;Møller BL;Gorodkin J;Bak S
植物和昆虫共同进化的一个重要驱动因素是产生和处理生物活性化合物的能力。植物产生生物活性天然产物用于防御，但一些昆虫解毒和/或隔离化合物，开辟了竞争对手较少的新利基市场。为了研究植物-昆虫互作中共适应的分子机制，以百脉根（Lotus corniculatus）和细摆结缕草（Zygaena filipendulae）为材料，研究了它们之间的互作关系。它们都含有产氰糖苷，分解时释放有毒的氰化氢。属于Zygaena家族的蛾是唯一已知的昆虫，能够进行与来自其饲料植物的相同的生氰葡糖苷的从头生物合成和螯合。研究了Z.丝状真菌利用与植物中已知途径相同的中间体进行，但没有鉴定出相关的酶。一个基因组学策略建立在454焦磷酸测序的Z。filiacrylae转录组进行鉴定的Z.丝状菌。Z的比较。filiorae转录组与家蚕、黑腹果蝇、赤拟谷盗、意大利蜜蜂和冈比亚按蚊的基因组序列比较表明，Z.丝状真菌转录组11%的Z。将丝状真菌转录组序列分配到基因本体论类别。根据序列长度、拷贝数和D. melanogaster，B. B.和生氰蝴蝶Heliconius。偏密码子的使用，GC含量和基因候选人的选择检查支持的概念，蓝藻作为一个“老”性状内Ditrysia，以及其起源之间的收敛植物和昆虫。焦磷酸测序是获得来自昆虫和其他生物的生物活性天然产物的生物合成中的基因的有吸引力的方法，其基因组序列未知。在分析Z.丝状菌转录组，有前途的基因候选人的生氰糖苷的生物合成进行了鉴定，和Z。丝状真菌作为昆虫发蓝的模式系统是显而易见的。
An essential driving component in the co-evolution of plants and insects is the ability to produce and handle bioactive compounds. Plants produce bioactive natural products for defense, but some insects detoxify and/or sequester the compounds, opening up for new niches with fewer competitors. To study the molecular mechanism behind the co-adaption in plant-insect interactions, we have investigated the interactions between Lotus corniculatus and Zygaena filipendulae. They both contain cyanogenic glucosides which liberate toxic hydrogen cyanide upon breakdown. Moths belonging to the Zygaena family are the only insects known, able to carry out both de novo biosynthesis and sequestration of the same cyanogenic glucosides as those from their feed plants. The biosynthetic pathway for cyanogenic glucoside biosynthesis in Z. filipendulae proceeds using the same intermediates as in the well known pathway from plants, but none of the enzymes responsible have been identified. A genomics strategy founded on 454 pyrosequencing of the Z. filipendulae transcriptome was undertaken to identify some of these enzymes in Z. filipendulae. Comparisons of the Z. filipendulae transcriptome with the sequenced genomes of Bombyx mori, Drosophila melanogaster, Tribolium castaneum, Apis mellifera and Anopheles gambiae indicate a high coverage of the Z. filipendulae transcriptome. 11% of the Z. filipendulae transcriptome sequences were assigned to Gene Ontology categories. Candidate genes for enzymes functioning in the biosynthesis of cyanogenic glucosides (cytochrome P450 and family 1 glycosyltransferases) were identified based on sequence length, number of copies and presence/absence of close homologs in D. melanogaster, B. mori and the cyanogenic butterfly Heliconius. Examination of biased codon usage, GC content and selection on gene candidates support the notion of cyanogenesis as an "old" trait within Ditrysia, as well as its origins being convergent between plants and insects. Pyrosequencing is an attractive approach to gain access to genes in the biosynthesis of bio-active natural products from insects and other organisms, for which the genome sequence is not known. Based on analysis of the Z. filipendulae transcriptome, promising gene candidates for biosynthesis of cyanogenic glucosides was identified, and the suitability of Z. filipendulae as a model system for cyanogenesis in insects is evident.
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影响因子：
14.9
作者：
Bairoch, Amos;Bougueleret, Lydie;Zhang, Jian
通讯作者：
Zhang, Jian
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
DOI：
10.1016/0305-0491(83)90041-x
发表时间：
1983-01-01
影响因子：
2.2
作者：
NAHRSTEDT, A;DAVIS, RH
通讯作者：
DAVIS, RH
DOI：
10.1016/0020-1790(87)90037-0
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
DAVIS, RH;NAHRSTEDT, A
通讯作者：
NAHRSTEDT, A
DOI：
10.1073/pnas.0812141106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Chung, Henry;Sztal, Tamar;Daborn, Phillip J.
通讯作者：
Daborn, Phillip J.