Differential relative sulfation of Keratan sulfate glycosaminoglycan in the chick cornea during embryonic development.
Differential relative sulfation of Keratan sulfate glycosaminoglycan in the chick cornea during embryonic development.
复制标题
胚胎发育过程中鸡角膜中硫酸角质素糖胺聚糖的相对硫酸化差异。
DOI：
10.1167/iovs.09-4004
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发表时间：
2010
影响因子：
4.4
通讯作者：
Liles M
中科院分区：
文献类型：
--
作者：
Liles M
目的：研究胚胎发生和角膜退化发生过程中，伴随着硫酸角质素（KS）糖胺聚糖（GAG）表位硫酸化模式改变的发育中角膜基质的结构重塑。从孵育的胚胎第12天至E18天获得发育中的鸡角膜。通过同步加速器X射线衍射、羟脯氨酸测定、ELISA（使用针对较少和较高硫酸化KS的抗体）和透射电子显微镜用特异性蛋白聚糖染色来评估细胞外基质成分和胶原纤维间距。一个显着的相对增加高度硫酸化KS表位标记发生相对于羟脯氨酸含量在最后一周的鸡发展，平均胶原纤维间距离下降。小KS PG细丝的频率随着发育而增加，并且主要与原纤维相关。在E12至E18时间范围内高度硫酸化KS的积累可以用于在角膜生长期间微调局部基质水合和胶原纤维间距，因为通过新生内皮泵的作用，基质的总体脱水和压实进展。
Purpose.: To investigate structural remodeling of the developing corneal stroma concomitant with changing sulfation patterns of keratan sulfate (KS) glycosaminoglycan (GAG) epitopes during embryogenesis and the onset of corneal transparency.Methods.: Developing chick corneas were obtained from embryonic day (E) 12 to E18 of incubation. Extracellular matrix composition and collagen fibril spacing were evaluated by synchrotron x-ray diffraction, hydroxyproline assay, ELISA (with antibodies against lesser and more highly sulfated KS), and transmission electron microscopy with specific proteoglycan staining.Results.: A significant relative increase in highly sulfated KS epitope labeling occurred with respect to hydroxyproline content in the final week of chick development, as mean collagen interfibrillar distance decreased. Small KS PG filaments increased in frequency with development and were predominantly fibril associated.Conclusions.: The accumulation of highly sulfated KS during the E12 to E18 timeframe could serve to fine tune local matrix hydration and collagen fibril spacing during corneal growth, as gross dehydration and compaction of the stroma progress through the action of the nascent endothelial pump.
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影响因子：
17.8
作者：
Linsenmayer,TF;Fitch,JM;Gordon,MK;Cai,CX;Igoe,F;Marchant,JK;Birk,DE
通讯作者：
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影响因子：
4.4
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影响因子：
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作者：
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影响因子：
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