MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1 alpha.
MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1 alpha.
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DOI：
10.1080/21655979.2022.2063537
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发表时间：
2022-04
期刊：
影响因子：
4.9
通讯作者：
中科院分区：
文献类型：
--
作者：
肺腺癌是一种严重威胁人类健康的恶性肿瘤。然而，土地利用变化与发展的内在机制在很大程度上仍不清楚。本研究旨在探讨miR-218- 5 p在LUAD中的功能。MiR-218- 5 p和内质网氧化还原酶1 α（ERO 1A）通过生物信息学分析分别被筛选为LUAD中不同下调和上调的RNA。细胞功能分析的结果表明，miR-218- 5 p的强制表达显著抑制LUAD中的细胞活力、侵袭和迁移。生物信息学分析表明，miR-218- 5 p可能与ERO 1A mRNA的3 '端非翻译区相互作用。随后，采用western blot和双荧光素酶报告基因分析来鉴定它们之间的相互作用。ERO 1A过表达逆转了miR-218- 5 p对LUAD细胞进展的抑制作用，表明miR-218- 5 p/ERO 1A轴在抑制癌症发展中的意义。我们还观察到，这个调节轴抑制血管生成LUAD。因此，miR-218- 5 p/ERO 1A轴在LUAD细胞的生长过程中起重要作用，为LUAD治疗方案的制定提供了有价值的线索。
Lung adenocarcinoma (LUAD) severely threatens the health of people owing to its lethality. Nonetheless, the underlying mechanisms on LUAD development remain unclear to a great extent. This work aimed to probe the functions of miR-218-5p in LUAD. MiR-218-5p and endoplasmic reticulum oxidoreductase 1 alpha (ERO1A) were screened as differently downregulated and upregulated RNAs in LUAD, respectively, by bioinformatics analyses. The results of cell functional assays stated that enforced expression of miR-218-5p notably restrained cell viability, invasion, and migration in LUAD. MiR-218-5p may interact with 3’-untranslated region of ERO1A mRNA as analyzed by bioinformatics. Afterward, western blot and dual-luciferase reporter gene analyses were introduced to identify their interaction. ERO1A overexpression reversed the suppressive impacts of miR-218-5p on LUAD cell progression, indicating the implication of miR-218-5p/ERO1A axis in suppressing cancer development. We also observed that this regulatory axis suppressed angiogenesis in LUAD. Taken together, miR-218-5p/ERO1A axis exerted an imperative role in LUAD cell progression, which provides a valuable clue for the development of LUAD therapeutic regimen.
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