Accumulation of Ca(v)3.2 T-type Calcium Channels in the Uninjured Sural Nerve Contributes to Neuropathic Pain in Rats with Spared Nerve Injury.
Accumulation of Ca(v)3.2 T-type Calcium Channels in the Uninjured Sural Nerve Contributes to Neuropathic Pain in Rats with Spared Nerve Injury.
复制标题
未损伤腓肠神经中 Ca(v)3.2 T 型钙通道的积累导致神经损伤大鼠的神经病理性疼痛
DOI：
10.3389/fnmol.2018.00024
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发表时间：
2018
影响因子：
4.8
通讯作者：
Liu FY
中科院分区：
文献类型：
--
作者：
Chen W;Chi YN;Kang XJ;Liu QY;Zhang HL;Li ZH;Zhao ZF;Yang Y;Su L;Cai J;Liao FF;Yi M;Wan Y;Liu FY
周围神经纤维的损伤会引起神经病理性疼痛。但是，相邻的未损伤的纤维与疼痛的关系尚不完全清楚。本研究旨在探讨未损伤的传入神经纤维中Cav3.2T型钙通道在备用神经损伤(SNI)大鼠神经病理性疼痛中的可能作用。在体单单位记录显示未损伤腓肠神经的β、A、δ和C纤维致敏，Western blotting显示伴有Cav3.2T型钙通道蛋白的积聚。将T型钙通道阻滞剂米贝法地尔应用于腓肠神经感受区，可提高Aβ、Aδ和C纤维的机械阈值，证实SNI大鼠腓肠神经聚集通道的功能受累。最后，在未损伤的腓肠神经周围应用米贝地尔或TTA-P2可减轻SNI大鼠的机械性痛觉异常。这些结果提示，Cav3.2T型钙通道的轴突聚集在未损伤的腓肠神经敏化中起重要作用，并参与神经病理性疼痛。
Injuries to peripheral nerve fibers induce neuropathic pain. But the involvement of adjacent uninjured fibers to pain is not fully understood. The present study aims to investigate the possible contribution of Cav3.2 T-type calcium channels in uninjured afferent nerve fibers to neuropathic pain in rats with spared nerve injury (SNI). Aβ-, Aδ- and C-fibers of the uninjured sural nerve were sensitized revealed by in vivo single-unit recording, which were accompanied by accumulation of Cav3.2 T-type calcium channel proteins shown by Western blotting. Application of mibefradil, a T-type calcium channel blocker, to sural nerve receptive fields increased mechanical thresholds of Aβ-, Aδ- and C-fibers, confirming the functional involvement of accumulated channels in the sural nerve in SNI rats. Finally, perineural application of mibefradil or TTA-P2 to the uninjured sural nerve alleviated mechanical allodynia in SNI rats. These results suggest that axonal accumulation of Cav3.2 T-type calcium channels plays an important role in the uninjured sural nerve sensitization and contributes to neuropathic pain.
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