Complex genetic interactions underlying expression differences between Drosophila races: Analysis of chromosome substitutions
Complex genetic interactions underlying expression differences between Drosophila races: Analysis of chromosome substitutions
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果蝇种族之间表达差异背后的复杂遗传相互作用：染色体替换分析
DOI：
10.1073/pnas.0711774105
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发表时间：
2008-04
影响因子：
11.1
通讯作者：
Wu, Mao-Lien
中科院分区：
文献类型：
--
作者：
Lu, Jian;Lu, Xuemei;McPeek, Mary Sara;Xu, Anlong;Nuzhdin, Sergey;Wu, Chung-I;Wang, Hurng-Yi;Fu, Yonggui;Wu, Mao-Lien
基因表达的调控通常分为顺式和反式两部分。如果表达中的许多变异是多基因和上位性的（例如，顺式-反式）对照。因此，有必要量化的相对贡献的顺式，反式，顺式反式效应表达分歧在不同的进化水平。为了在整个基因组中做到这一点，我们分析了黑腹果蝇两个行为种族之间的全套染色体置换系。我们的观察：（i）只有约3%的基因表达差异是纯粹的顺式调节。事实上，相对较少的基因是由简单的遗传学控制的，因为近80%的表达差异至少由两条染色体控制。(ii)对于14%的基因，顺式调节确实起作用，但通常与反式调节结合。这种顺式和反式效应的联合作用，无论是加性的还是上位性的，都被称为包容性顺式效应。(iii)在两个小种间差异显著的基因中，具有包容性顺式效应的基因比例增加到32%。(iv)我们观察到反式作用因子的非随机分布，在第二条染色体上存在大量缺陷。在果蝇种族群体之间，表达差异的反式调节是广泛的，顺式调节经常与反式效应一起进化。
Regulation of gene expression is usually separated into cis and trans components. The separation may become artificial if much of the variation in expression is under multigenic and epistatic (e.g., cis-by-trans) control. There is hence a need to quantify the relative contribution of cis, trans, and cis-by-trans effects on expression divergence at different levels of evolution. To do so across the whole genome, we analyzed the full set of chromosome-substitution lines between the two behavioral races of Drosophila melanogaster. Our observations: (i) Only ≈3% of the genes with an expression difference are purely cis regulated. In fact, relatively few genes are governed by simple genetics because nearly 80% of expression differences are controlled by at least two chromosomes. (ii) For 14% of the genes, cis regulation does play a role but usually in conjunction with trans regulation. This joint action of cis and trans effects, either additive or epistatic, is referred to as inclusive cis effect. (iii) The percentage of genes with inclusive cis effect increases to 32% among genes that are strongly differentiated between the two races. (iv) We observed a nonrandom distribution of trans-acting factors, with a substantial deficit on the second chromosome. Between Drosophila racial groups, trans regulation of expression difference is extensive, and cis regulation often evolves in conjunction with trans effects.
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影响因子：
56.9
作者：
Parisi, M;Nuttall, R;Oliver, B
通讯作者：
Oliver, B
影响因子：
10.7
作者：
Osada, Naoki;Kohn, Michael H.;Wu, Chung-I
通讯作者：
Wu, Chung-I
影响因子：
30.8
作者：
Cowles, CR;Hirschhorn, JN;Lander, ES
通讯作者：
Lander, ES
影响因子：
2.9
作者：
Alipaz, JA;Fang, S;Wu, CI
通讯作者：
Wu, CI
影响因子：
--
作者：
Gupta V;Parisi M;Sturgill D;Nuttall R;Doctolero M;Dudko OK;Malley JD;Eastman PS;Oliver B
通讯作者：
Oliver B