The Role of sho1 in Polarized Growth of Aspergillus fumigatus
The Role of sho1 in Polarized Growth of Aspergillus fumigatus
复制标题
sho1在烟曲霉极化生长中的作用
DOI：
10.1007/s11046-011-9452-4
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发表时间：
2011-07
期刊：
影响因子：
5.5
通讯作者：
Li, Ruoyu
中科院分区：
文献类型：
--
作者：
Yang, Fang;Ma, Dongmei;Wan, Zhe;Liu, Wei;Ji, Yajuan;Li, Ruoyu
烟曲霉菌是一种机会致病菌，可引起免疫功能低下患者严重的侵袭性疾病。丝状真菌经历极化生长，在环境中寻找营养物质并在组织中引起侵入性生长。Sho 1是高渗透压甘油途径的传感器，并且Sho 1突变体显示生长速率降低。我们发现sho 1参与了A.烟熏。荧光素白色染色显示sho 1突变导致萌发分生孢子的各向同性生长延长，随后出现多个芽管和宽的菌丝以及短的居间细胞。探讨了sho 1基因影响极化生长的机制。通过透射电子显微镜观察到的sho 1突变体的Spitzenkörper体的顶端囊泡的数量减少，分散度更大。肌动蛋白斑块在突变菌株真菌发育的早期阶段以低密度随机分布，并在长丝状真菌形成的后期阶段重新聚集到菌丝顶端。位于极化野生型细胞顶端的肌动蛋白斑块。实时荧光定量PCR检测极化生长相关基因Rho GTPases的RNA水平。菌株在37°C培养6 h后，sho 1基因对RNA表达没有影响。在17 h时，rho 1，rho 3和CDC 42在sho 1突变体中的RNA表达分别是野生型的0.18-，0.18-和0.33-倍。sho 1基因通过影响Rho GTPases的表达、肌动蛋白骨架的分布、囊泡的数量和分布来影响极化生长。
Aspergillus fumigatus is an opportunistic pathogen that may cause severe invasive disease in immunocompromised patients. The filamentous fungi undergo polarized growth, searching for nutrients in the environment and causing invasive growth in tissue. Sho1 is a sensor of the high osmolarity glycerol pathway, and the sho1 mutant showed a decrease in growth rate. We found that sho1 is involved in the polarized growth of A. fumigatus. The sho1 mutation resulted in extended isotropic growth of germinating conidia followed by multiple germ tubes and wide hyphae with short intercalary cells by calcofluor white staining. The mechanism by which sho1 gene affected polarized growth is investigated. A reduced number of apical vesicles with greater dispersion were observed by transmission electron microscopy in the Spitzenkörper body of the sho1 mutant. Actin patches were distributed randomly at low density at early stages of mutant strain fungal development and reaggregated to the hyphal tip of later stages when long filamentous fungi formed. Actin patches located at the tip of polarized wild-type cells. RNA levels of polarized growth-related genes Rho GTPases were detected by real-time PCR. The sho1 gene did not affect the RNA expression when strains were cultured at 37°C for 6 h. At 17 h, the RNA expression of rho1, rho3 and CDC42 in the sho1 mutant were 0.18-, 0.18- and 0.33-fold of that in the wild type. The sho1 gene affected the polarized growth through affecting the expression of Rho GTPases, the distribution of actin cytoskeleton, vesicle quantity and distribution.
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影响因子：
21.3
作者：
V. Reiser;Suhal M. Salah;G. Ammerer
通讯作者：
V. Reiser;Suhal M. Salah;G. Ammerer
影响因子：
4.8
作者：
Garcia, Raul;Rodriguez-Pena, Jose M.;Arroyo, Javier
通讯作者：
Arroyo, Javier
影响因子：
3.6
作者：
Huijun Wei;N. Requena;R. Fischer
通讯作者：
Huijun Wei;N. Requena;R. Fischer
影响因子：
3.3
作者：
T. Yuzyuk;David C. Amberg
通讯作者：
T. Yuzyuk;David C. Amberg
DOI：
10.1016/s1087-1845(02)00506-6
发表时间：
2002-11
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
M. Riquelme;R. Roberson;D. McDaniel;S. Bartnicki-García
通讯作者：
M. Riquelme;R. Roberson;D. McDaniel;S. Bartnicki-García