Biochemical characterization of metabolism-based atrazine resistance in Amaranthus tuberculatus and identification of an expressed GST associated with resistance.
Biochemical characterization of metabolism-based atrazine resistance in Amaranthus tuberculatus and identification of an expressed GST associated with resistance.
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DOI：
10.1111/pbi.12711
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发表时间：
2017-10
影响因子：
13.8
通讯作者：
Riechers DE
中科院分区：
文献类型：
--
作者：
Evans AF Jr;O'Brien SR;Ma R;Hager AG;Riggins CW;Lambert KN;Riechers DE
阿特拉津在天然耐受作物如玉米（Zea mays）和高粱（Sorghum bicolor）中的快速解毒作用来自谷胱甘肽S-转移酶（GST）活性。在之前的研究中，来自美国伊利诺伊州的两个阿特拉津耐药性水麻（Amaranthus tuberculatus）种群（指定为ACR和MCR）显示阿特拉津-谷胱甘肽（GSH）结合物的快速形成，表明代谢率升高是耐药性机制。我们的主要目的是利用蛋白质纯化结合定性蛋白质组学研究的假设，增强阿特拉津解毒，催化不同的GST，赋予耐药性ACR和MCR。此外，在分离阿特拉津抗性的F2群体中分析候选AtuGST表达。与阿特拉津敏感人群（WCS）相比，ACR和MCR在部分纯化的硫酸铵和GSH亲和纯化组分中对阿特拉津显示出更高的比活性。这些组分的一维电泳显示约26 kDa的条带，是GST亚基的典型条带。基于与拟南芥GST的肽相似性，通过LC-MS/MS从每个群体中鉴定出几种phi和tau类GST。一种phi类GST（命名为AtuGSTF 2）的组成型表达升高与ACR和MCR以及分离F2群体中的阿特拉津抗性密切相关。这些结果表明AtuGSTF 2可能与ACR和MCR中赋予阿特拉津抗性的代谢机制有关。
Rapid detoxification of atrazine in naturally tolerant crops such as maize (Zea mays) and grain sorghum (Sorghum bicolor) results from glutathione S‐transferase (GST) activity. In previous research, two atrazine‐resistant waterhemp (Amaranthus tuberculatus) populations from Illinois, U.S.A. (designated ACR and MCR), displayed rapid formation of atrazine‐glutathione (GSH) conjugates, implicating elevated rates of metabolism as the resistance mechanism. Our main objective was to utilize protein purification combined with qualitative proteomics to investigate the hypothesis that enhanced atrazine detoxification, catalysed by distinct GSTs, confers resistance in ACR and MCR. Additionally, candidate AtuGST expression was analysed in an F2 population segregating for atrazine resistance. ACR and MCR showed higher specific activities towards atrazine in partially purified ammonium sulphate and GSH affinity‐purified fractions compared to an atrazine‐sensitive population (WCS). One‐dimensional electrophoresis of these fractions displayed an approximate 26‐kDa band, typical of GST subunits. Several phi‐ and tau‐class GSTs were identified by LC‐MS/MS from each population, based on peptide similarity with GSTs from Arabidopsis. Elevated constitutive expression of one phi‐class GST, named AtuGSTF2, correlated strongly with atrazine resistance in ACR and MCR and segregating F2 population. These results indicate that AtuGSTF2 may be linked to a metabolic mechanism that confers atrazine resistance in ACR and MCR.
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影响因子：
4.7
作者：
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通讯作者：
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