Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response.
Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response.
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DOI：
10.3390/cells10113073
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发表时间：
2021-11-08
期刊：
影响因子：
6
通讯作者：
Batista ML Jr
中科院分区：
文献类型：
--
作者：
Biagi CAO Jr;Cury SS;Alves CP;Rabhi N;Silva WA Jr;Farmer SR;Carvalho RF;Batista ML Jr
脂肪组织根据其形态和功能分为白色、棕色或米色/浅褐色。它通过旁分泌和内分泌信号作为全身代谢的调节剂发挥着重要作用。最近，使用RNA测序技术揭示了多种脂肪细胞亚型，不仅限于简单定义的形态，还包括它们的细胞起源、对代谢应激的适应和可塑性。在这里，我们对来自脂肪组织的公开单核 RNAseq 进行了深入分析，并利用工作流程模板来表征脂肪细胞的可塑性、异质性和分泌组概况。重新分析的数据集导致识别出不同的脂肪细胞亚型，包括三个产热脂肪细胞亚群，并提供了在产热挑战下沿着脂肪细胞轨迹的不同转录谱的特征。这项研究为进一步研究脂肪细胞可塑性和转分化相关机制提供了有用的资源。
Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.
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