MaRAP2-4, a waterlogging-responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis.
MaRAP2-4, a waterlogging-responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis.
复制标题
MARAP2-4是Mentha的水槽响应ERF，调节双向糖转运蛋白ATSWEET10，以调节拟南芥中的应力反应。
DOI：
10.1111/pbi.12762
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发表时间：
2018-01
影响因子：
13.8
通讯作者：
Shukla RK
中科院分区：
文献类型：
--
作者：
Phukan UJ;Jeena GS;Tripathi V;Shukla RK
由于水涝和连续事件严重影响经济上重要的植物的生长和发育，我们试图表征来自薄荷的水涝响应组I（A-6）乙烯响应因子（MaRAP 2 - 4）的作用。淹水、乙烯和茉莉酸甲酯快速诱导MaRAP 2 - 4的表达。MaRAP 2 - 4与脱水反应元件（DRE 1/2）、缺氧/茉莉酸反应元件（JARE）和GCC盒等多个顺式元件相互作用，显示其参与多种反应。MaRAP 2 ‐4定位于细胞核中，并作为转录激活因子。截短和内部缺失鉴定了MaRAP 2 - 4中20个氨基酸的潜在反式激活结构域（PLPSSVDAKLEAICQSLAIN）。MaRAP 2 - 4转基因拟南芥表现出增强的水涝和随后的氧化胁迫耐受性。微阵列分析显示，在上调基因中，483、212和132个启动子分别携带DRE、JARE和GCC顺式元件的单个或多个拷贝。在这些启动子中，大部分属于碳水化合物代谢/转运，包括许多SWEET转运蛋白。进一步的分析表明，MaRAP 2 - 4特异性靶向AtSWEEET 10启动子中携带DRE和/或GCC盒的两个位置，这些位置可能调节碳水化合物的可用性和耐涝性。这些结果表明，MaRAP 2 - 4是耐涝性的正调节因子，并且由于能量消耗过程（如碳水化合物生物合成）在涝渍诱导的缺氧下减少，通过SWEET的糖转运可能是使糖可用于所需组织的主要选择。
As waterlogging and successive events severely influence growth and development of economically important plants, we attempted to characterize the role of a waterlogging‐responsive group I (A‐6) ethylene response factor (MaRAP2‐4) from Mentha arvensis. Waterlogging, ethylene and methyl jasmonate rapidly induced the expression of MaRAP2‐4. MaRAP2‐4 interacted with multiple cis‐elements like dehydration response elements (DRE1/2), anoxia/jasmonic acid response element (JARE) and GCC box showing its involvement in multiple responses. MaRAP2‐4 localizes in the nucleus and acts as a transcriptional activator. Truncation and internal deletion identified a 20 amino acids potential transactivation domain (PLPSSVDAKLEAICQSLAIN) in MaRAP2‐4. MaRAP2‐4 transgenic Arabidopsis showed enhanced waterlogging and subsequent oxidative stress tolerance. Microarray analysis revealed that within up‐regulated genes 483, 212 and 132 promoters carry either single or multiple copies of DRE, JARE and GCC cis‐element/s, respectively. Within these promoters, a large section belongs to carbohydrate metabolism/transport, including many SWEET transporters. Further analysis showed MaRAP2‐4 specifically targets two positions in AtSWEEET10 promoter carrying DRE and/or GCC box that might regulate carbohydrate availability and waterlogging tolerance. These results demonstrate that MaRAP2‐4 is a positive regulator of waterlogging tolerance, and as energy‐consuming processes such as carbohydrate biosynthesis are reduced under waterlogging‐induced hypoxia, sugar transport through SWEETs may be the primary option to make sugar available to the required tissue.
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