Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells.
Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells.
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DOI：
10.3892/ijo_00000710
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发表时间：
2010-09
影响因子：
5.2
通讯作者：
Kieber-Emmons T
中科院分区：
文献类型：
--
作者：
Monzavi-Karbassi B;Hine RJ;Stanley JS;Ramani VP;Carcel-Trullols J;Whitehead TL;Kelly T;Siegel ER;Artaud C;Shaaf S;Saha R;Jousheghany F;Henry-Tillman R;Kieber-Emmons T
异常糖基化是癌细胞的普遍特征，并且某些聚糖结构是肿瘤进展的众所周知的标志物。微环境中糖的可用性和组成可能影响细胞糖基化。最近对人类乳腺肿瘤细胞系的研究表明它们具有摄取和利用果糖的能力。在这里，我们测试的假设，即添加果糖作为碳源培养诱导培养的人乳腺癌细胞的表型变化，与转移性疾病。使MDA-MB-468细胞适应其中果糖取代葡萄糖的培养基。测定了细胞表面聚糖结构的变化、与聚糖组装相关的基因表达、细胞骨架F-肌动蛋白、迁移、粘附和侵袭。在果糖中培养的细胞表达不同的细胞表面聚糖。果糖的添加影响唾液酸化和岩藻糖基化模式。果糖喂养也增加了白细胞凝集菜豆异凝集素的结合，表明分支β-1，6 GlcNAc结构的表达可能增加。罗丹明鬼笔环肽染色显示改变的F-肌动蛋白细胞骨架系统。果糖加速细胞迁移和增加侵袭。这些数据表明，改变侵袭性较低的MDA-MB-468细胞系的碳源诱导了与更具侵袭性的表型相关的特征。由于近年来含有游离果糖的甜味剂的消费量显着增加，这些数据可能具有根本的重要性，因为它们表明果糖在肿瘤细胞的营养微环境中的存在可能会对一些乳腺癌患者的结果产生负面影响。
Aberrant glycosylation is a universal feature of cancer cells, and certain glycan structures are well-known markers for tumor progression. Availability and composition of sugars in the microenvironment may affect cell glycosylation. Recent studies of human breast tumor cell lines indicate their ability to take up and utilize fructose. Here we tested the hypothesis that adding fructose to culture as a carbon source induces phenotypic changes in cultured human breast tumor cells that are associated with metastatic disease. MDA-MB-468 cells were adapted to culture media in which fructose was substituted for glucose. Changes in cell surface glycan structures, expression of genes related to glycan assembly, cytoskeleton F-actin, migration, adhesion and invasion were determined. Cells cultured in fructose expressed distinct cell-surface glycans. The addition of fructose affected sialylation and fucosylation patterns. Fructose feeding also increased binding of leukoagglutinating Phaseolus vulgaris isolectin, suggesting a possible rise in expression of branching β-1, 6 GlcNAc structures. Rhodamine-phalloidin staining revealed an altered F-actin cytoskeletal system. Fructose accelerated cellular migration and increased invasion. These data suggest that changing the carbon source of the less aggressive MDA-MB-468 cell line induced characteristics associated with more aggressive phenotypes. These data could be of fundamental importance due to the markedly increased consumption of sweeteners containing free fructose in recent years, as they suggest that the presence of fructose in nutritional micro-environment of tumor cells may negatively affect the outcome for some breast cancer patients.
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DOI：
10.1007/bf02618875
发表时间：
1984-01-01
期刊：
IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION
影响因子：
--
作者：
DELHOTAL, B;LEMONNIER, F;LEMONNIER, A
通讯作者：
LEMONNIER, A
影响因子：
11.5
作者：
Handerson, T;Camp, R;Pawelek, J
通讯作者：
Pawelek, J
DOI：
10.1186/bcr75
发表时间：
2000
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Couldrey C;Green JE
通讯作者：
Green JE
影响因子：
4
作者：
Kelly, T;Yan, Y;Chu, WS
通讯作者：
Chu, WS
影响因子：
3
作者：
Mack, DR;Cheng, PW;Hollingsworth, MA
通讯作者：
Hollingsworth, MA